Isoflurane attenuates blood-brain barrier disruption in ipsilateral hemisphere after subarachnoid hemorrhage in mice.
Isoflurane attenuates blood-brain barrier disruption in ipsilateral hemisphere after subarachnoid hemorrhage in mice.
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DOI：
10.1161/strokeaha.112.661728
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发表时间：
2012-09
期刊：
影响因子：
8.3
通讯作者：
Zhang JH
中科院分区：
文献类型：
--
作者：
Altay O;Suzuki H;Hasegawa Y;Caner B;Krafft PR;Fujii M;Tang J;Zhang JH
研究了挥发性麻醉剂异氟醚对小鼠蛛网膜下腔出血（SAH）血管内穿孔模型血脑屏障（BBB）破坏的影响。动物被分配到假手术组、SAH+车辆-空气组、SAH+1%或2%异氟醚组。在sah后24小时评估神经行为功能、脑含水量、Evans蓝染料外渗和鞘磷脂激酶（SphKs）、occludin、cludin -5、连接粘附分子和血管内皮钙粘蛋白的Western印迹。还考察了SphK （DMS）或鞘氨醇-1-磷酸受体-1/3 （S1P1/3）抑制剂（VPC23019）对异氟烷作用的影响。SAH加重了神经学评分、脑水肿和血脑屏障通透性，治疗后2%异氟醚可预防这些症状，但1%异氟醚不能预防。2%异氟烷增加SphK1的表达，并阻止sah后bbb相关蛋白表达的下降。DMS和VPC23019都消除了异氟烷的有益作用。2%异氟醚可以抑制sah后血脑屏障的破坏，这可能是由SphK1表达和S1P1/3激活介导的。
We examined effects of isoflurane, volatile anesthetics, on blood-brain barrier (BBB) disruption in the endovascular perforation model of subarachnoid hemorrhage (SAH) in mice. Animals were assigned to sham-operated, SAH+vehicle-air, SAH+1% or 2% isoflurane groups. Neurobehavioral function, brain water content, Evans blue dye extravasation and Western blotting for sphingosine kinases (SphKs), occludin, claudin-5, junctional adhesion molecule and vascular endothelial cadherin were evaluated at 24 hours post-SAH. Effects of SphK (DMS) or sphingosine-1-phosphate receptor-1/3 (S1P1/3) inhibitors (VPC23019) on isoflurane's action were also examined. SAH aggravated neurological scores, brain edema and BBB permeability, which were prevented by 2% but not 1% isoflurane posttreatment. 2% isoflurane increased SphK1 expression and prevented a post-SAH decrease in expressions of the BBB-related proteins. Both DMS and VPC23019 abolished the beneficial effects of isoflurane. 2% isoflurane can suppress post-SAH BBB disruption, which may be mediated by SphK1 expression and S1P1/3 activation.