Modulation of sodium-channel mRNA levels in rat skeletal muscle.
Modulation of sodium-channel mRNA levels in rat skeletal muscle.
复制标题
大鼠骨骼肌中钠通道 mRNA 水平的调节。
DOI：
10.1073/pnas.84.23.8721
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发表时间：
1987
影响因子：
11.1
通讯作者：
Mandel,G
中科院分区：
文献类型：
--
作者：
Cooperman,SS;Grubman,SA;Barchi,RL;Goodman,RH;Mandel,G
许多可兴奋细胞的动作电位是由其对钠离子的渗透性变化引起的。尽管神经和肌肉的这些通透性变化是由功能相似的电压门控钠通道介导的，但我们发现骨骼肌中表达的Na通道基因不同于大脑中编码两种Na通道（I型和II型）的基因。尽管肌肉和大脑na通道基因在结构上存在差异，但来自大鼠脑的cDNA克隆与骨骼肌na通道mRNA杂交约9.5千碱基。我们使用这种cDNA探针来测量骨骼肌在发育期间和去神经支配后na通道mRNA水平的变化。通过电泳分离RNA的印迹杂交分析，我们发现早在胚胎第17天就可以检测到na通道mRNA，并且在出生和出生后第35天之间mRNA水平增加了2倍。成人肌肉去神经支配导致肌肉Na-channel mRNA水平进一步增加2- 3倍，提示快缩肌Na-channel基因的表达可能受到神经支配状态的调节。
Action potentials in many types of excitable cells result from changes in permeability to Na ions. Although these permeability changes in nerve and muscle are mediated by voltage-gated Na channels that are functionally similar, we found that the Na-channel gene expressed in skeletal muscle is different from the genes coding for two Na channels (type I and type II) in brain. Despite the structural differences between muscle and brain Na-channel genes, a cDNA clone derived from rat brain hybridizes to skeletal muscle Na-channel mRNA of approximately 9.5 kilobases. We used this cDNA probe to measure changes in Na-channel mRNA levels in skeletal muscle during development and following denervation. By blot hybridization analysis of electrophoretically fractionated RNA, we found that Na-channel mRNA can be detected as early as embryonic day 17 and that mRNA levels increase 2-fold between birth and postnatal day 35. Denervation of adult muscle causes a further 2- to 3-fold increase in muscle Na-channel mRNA levels, suggesting that expression of Na-channel genes in fast-twitch muscle may be regulated by the state of innervation.