In vivo exit of c-kit+/CD49d(hi)/beta7+ mucosal mast cell precursors from the bone marrow following infection with the intestinal nematode Trichinella spiralis.
In vivo exit of c-kit+/CD49d(hi)/beta7+ mucosal mast cell precursors from the bone marrow following infection with the intestinal nematode Trichinella spiralis.
复制标题
体内感染肠道线虫旋毛虫后，c-kit /CD49d(hi)/β7 粘膜肥大细胞前体从骨髓中排出。
DOI：
10.1182/blood-2003-09-3146
复制
发表时间：
2004
期刊：
影响因子：
20.3
通讯作者：
R. Grencis
中科院分区：
文献类型：
--
作者：
J. Pennock;R. Grencis
我们已经使用寄生虫旋毛虫研究小鼠骨髓中肥大细胞祖细胞的产生和分化，因为这种感染引发与寄生虫排出相关的肠肥大细胞增多症。C-kit+肥大细胞祖细胞先前已通过甲基纤维素集落形成单位和体外有限稀释测定来定义。体内实验已经证明肥大细胞对特异性整合素表达的基本要求。我们在骨髓中确定了2个c-kit+群体，其中一个共表达CD 49 d/β 7整联蛋白，这是小肠迁移所必需的标志物。我们已经通过使用拮抗性抗c-kit抗体在体内证实了这些细胞的表型。我们的数据表明，c-kit+/β 7+细胞从骨髓中的损失与它们在血液中的出现相关，并且在肠道中检测到成熟肥大细胞之前3天。这种退出与血清中可溶性干细胞因子（SCF）的增加相关，这表明c-kit/SCF相互作用可能是趋化性或趋触性的。这项研究表明，在感染期间，骨髓环境产生肥大细胞，这些肥大细胞在进入外周之前进入肠粘膜，这表明感染部位和造血组织之间存在明显的相互作用。
We have used the parasite helminth Trichinella spiralis to study the generation and differentiation of mast cell progenitors in the bone marrow of mice, as this infection triggers an intestinal mastocytosis which correlates with parasite expulsion. C-kit+ mast cell progenitors have previously been defined by methylcellulose colony-forming units and by limiting dilution assays in vitro. In vivo experiments have demonstrated the essential requirement by mast cells for specific integrin expression. We have defined 2 c-kit+ populations in the bone marrow, one of which coexpresses CD49d/beta7 integrin, a marker essential for small intestine immigration. We have confirmed the phenotype of these cells by using antagonistic anti-c-kit antibody in vivo. Our data show that the loss of c-kit+/beta7+ cells from the bone marrow correlates with their appearance in the blood and precedes detection of mature mast cells in the gut by 3 days. This exit correlates with an increase in soluble stem cell factor (SCF) in the serum, suggesting that the c-kit/SCF interaction may be chemotactic or haptotactic in nature. This study shows that during infection the bone marrow environment generates mast cells destined for the intestinal mucosa before their exit into the periphery, indicating a clear interplay between infection site and hematopoietic tissue.
DOI：
--
发表时间：
1994-02
期刊：
The American journal of pathology
影响因子：
--
作者：
A. Iemura;M. Tsai;A. Ando;B. Wershil;S. Galli
通讯作者：
A. Iemura;M. Tsai;A. Ando;B. Wershil;S. Galli
c-Kit 和 alpha4 整合素介导的 IA 类 PI-3 激酶信号和 Rac 通路在肥大细胞中调节整合素定向迁移的收敛的遗传证据。
影响因子：
20.3
作者：
Tan,BaiLin;Yazicioglu,MustafaN;Ingram,David;McCarthy,Jennifer;Borneo,Jovencio;Williams,DavidA;Kapur,Reuben
通讯作者：
Kapur,Reuben