Isolation of a selenite-reducing and cadmium-resistant bacterium Pseudomonas sp strain RB for microbial synthesis of CdSe nanoparticles
Isolation of a selenite-reducing and cadmium-resistant bacterium Pseudomonas sp strain RB for microbial synthesis of CdSe nanoparticles
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DOI：
10.1016/j.jbiosc.2013.10.010
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发表时间：
2014-05-01
影响因子：
2.8
通讯作者：
Ike, Michihiko
中科院分区：
文献类型：
--
作者：
Ayano, Hiroyuki;Miyake, Masaki;Ike, Michihiko
从土壤样品中富集了能够从亚硒酸盐和镉离子合成 CdSe 的细菌。将土壤来源的细菌培养物重复转移到含有亚硒酸盐和镉离子的新培养基中42次（历时360天）后，获得了可以同时从液相中去除亚硒酸盐和镉离子（各1 mM）的富集培养物。能量色散 X 射线光谱 (EDS) 证实，培养物的颜色变成红棕色，表明产生了 CdSe 纳米颗粒。分离操作的结果是，最能合成 CdSe 的细菌被命名为假单胞菌 (Pseudomonas sp.)。获得RB。透射电子显微镜和EDS显示，当在相同的培养基中进行富集培养时，该菌株在细胞内部和细胞上积累了由硒和镉组成的纳米颗粒（10-20 nm）。该报告是第一篇描述亚硒酸盐还原和耐镉细菌的分离的报告。它对于在简单的单容器操作中合成 CdSe 纳米颗粒非常有用。 (C) 2013 年，日本生物技术协会。版权所有。
Bacteria capable of synthesizing CdSe from selenite and cadmium ion were enriched from a soil sample. After repeated transfer of the soil-derived bacterial cultures to a new medium containing selenite and cadmium ion 42 times (during 360 days), an enrichment culture that can simultaneously remove selenite and cadmium ion (1 mM each) from the liquid phase was obtained. The culture's color became reddish-brown, indicating CdSe nanoparticle production, as confirmed by energy-dispersive x-ray spectra (EDS). As a result of isolation operations, the bacterium that was the most responsible for synthesizing CdSe, named Pseudomonas sp. RB, was obtained. Transmission electron microscopy and EDS revealed that this strain accumulated nanoparticles (10-20 nm) consisting of selenium and cadmium inside and on the cells when cultivated in the same medium for the enrichment culture. This report is the first describing isolation of a selenite-reducing and cadmium-resistant bacterium. It is useful for CdSe nanoparticle synthesis in the simple one-vessel operation. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.