Construction of an infectious clone of simian foamy virus of Japanese macaque (SFVjm) and phylogenetic analyses of SFVjm isolates
Construction of an infectious clone of simian foamy virus of Japanese macaque (SFVjm) and phylogenetic analyses of SFVjm isolates
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日本猕猴猿泡沫病毒（SFVjm）感染性克隆的构建及SFVjm分离株的系统发育分析
DOI：
10.1016/j.gene.2014.07.025
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发表时间：
2014
期刊：
影响因子：
3.5
通讯作者：
Miyazawa T.
中科院分区：
文献类型：
--
作者：
Yoshikawa R;Nakagawa S;Okamoto M;Miyazawa T.
泡沫病毒属于逆转录病毒科的泡沫病毒属，已从许多哺乳动物中分离到。据报道，猴泡沫病毒（SFV）与宿主物种共同进化。本研究从日本本州岛的日本猕猴（Macaca fuscata）中分离到4株SFV（WK1、WK2、AR 1和AR 2），命名为SFVjm。我们构建了SFVjm株WK1的感染性分子克隆，命名为pJM 356。该克隆衍生的病毒在人（人胚肾293 T细胞）、非洲绿色猴（Vero细胞）和小鼠细胞系（Mus dunnitail成纤维细胞）中复制并诱导合胞体。系统发育分析还显示，这4个SFVjm菌株形成了两个不同的SFVjm簇。SFVjm株WK1和WK2与从台湾猕猴（Macaca cyclopis）分离的SFV形成一个簇，而株AR 1和AR 2与从恒河猴（Macaca mulatta）分离的SFV形成另一个簇。
Foamy viruses belong to the genusSpumavirusof the family Retroviridae and have been isolated from many mammalian species. It was reported that simian foamy viruses (SFVs) have co-evolved with host species. In this study, we isolated four strains (WK1, WK2, AR1 and AR2) of SFV (named SFVjm) from Japanese macaques (Macaca fuscata) in main island Honshu of Japan. We constructed an infectious molecular clone of SFVjm strain WK1, termed pJM356. The virus derived from the clone replicated and induced syncytia in human (human embryonic kidney 293T cells), African green monkey (Vero cells) and mouse cell lines (Mus dunnitail fibroblast cells). Phylogenetic analysis also revealed that these four SFVjm strains formed two distinct SFVjm clusters. SFVjm strains WK1 and WK2 and SFV isolated from Taiwanese macaques (Macaca cyclopis) formed one cluster, whereas strains AR1 and AR2 formed the other cluster with SFV isolated from a rhesus macaque (Macaca mulatta).