simple and sensitive Ce(OH)CO3/H2O2/TMB reaction system for colorimetric determination of H2O2 and glucose
simple and sensitive Ce(OH)CO3/H2O2/TMB reaction system for colorimetric determination of H2O2 and glucose
复制标题
简单灵敏的 Ce(OH)CO3/H2O2/TMB 反应系统，用于比色测定 H2O2 和葡萄糖
DOI：
10.1016/j.snb.2016.03.087
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发表时间：
2016
影响因子：
8.4
通讯作者：
Qin Weiping
中科院分区：
文献类型：
--
作者：
Zhang Xiang;Bi Xueqing;Di Weihua;Qin Weiping
H2O2的检测在生命活动、医疗诊断、工农业生产和环境监测等许多领域都至关重要。本工作开发了一种简单、灵敏的用于H2O2测定的两步反应体系Ce(OH)CO3/H2O2/TMB。将 H2O2 和 TMB 依次添加到 Ce(OH)CO3 粉末中后，很快就会发生典型的显色反应，在微酸性水溶液中产生特有的蓝色。基于模拟酶催化的显色反应提出了潜在的反应机理。颜色深度对 H2O2 浓度的依赖性使得 H2O2 的比色测定成为可能。该反应体系在0-80 μM的H2O2浓度范围内线性快速响应，检测限达到0.3 μM H2O2，相对标准偏差低于5.1%。由于 H2O2 是氧化酶催化的葡萄糖氧化反应的主要产物之一，因此对该 H2O2 传感系统进行了修改，可以检测葡萄糖。除了宽线性响应、低检测限和高重现性之外，我们目前的葡萄糖测定反应系统由于葡萄糖氧化酶对葡萄糖的特异性而表现出对葡萄糖的高度特异性响应。
The detection for H2O2is essential in many areas, including life activity, medical diagnosis, industry and agriculture production and environmental monitoring, etc. This work developed a simple and sensitive two-step reaction system Ce(OH)CO3/H2O2/TMB for H2O2determination. Upon sequential addition of H2O2and TMB to Ce(OH)CO3powders, a typical color reaction occurred quickly, producing a characteristic blue color in a slightly acidic aqueous solution. The underlying reaction mechanism was proposed based on the color reaction catalyzed by mimetic enzyme. The dependence of the color depth on H2O2concentration enabled the colorimetric determination of H2O2. This reaction system responds linearly and quickly in a wide H2O2concentration range of 0–80 μM, and achieves a detection limit of 0.3 μM H2O2and a relative standard deviation lower than 5.1%. This H2O2sensing system was modified to allow for the detection of glucose since H2O2is one of the main products in the oxidation reaction of glucose catalyzed by oxidase enzymes. In addition to a wide linear response, a low detection limit and a high reproducibility, our present reaction system for glucose determination showed a highly specific response to glucose due to the specificity of glucose oxidase to glucose.