Signaling pathways controlling induced resistance to insect herbivores in Arabidopsis
Signaling pathways controlling induced resistance to insect herbivores in Arabidopsis
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DOI：
10.1094/mpmi-20-11-1406
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发表时间：
2007-11-01
影响因子：
3.5
通讯作者：
Reymond, Philippe
中科院分区：
文献类型：
--
作者：
Bodenhausen, Natacha;Reymond, Philippe
昆虫攻击触发植物转录物水平的变化，主要是由茉莉酸（JA）介导的。乙烯（ET）、水杨酸（SA）和其他信号在这一反应中的含义尚不清楚，并通过含有昆虫和防御调节基因的微阵列进行监测。拟南芥突变体coil-1、ein2-1和sid2-1在JA、ET和SA信号通路上受损，受到特殊的小白菜白虫（Pieris rapae）和通用的埃及棉虫（Spodoptera littoralis）的攻击。结果表明，JA是控制两种昆虫对防御基因上调的主要信号，但仅在对油菜的反应中抑制转录本水平的变化。幼虫的生长受ja依赖性防御的影响，但滨夜蛾在卷1上的增重明显大于油菜。ET和SA突变体的转录谱在取食海苔后发生改变，而在取食油菜后没有改变。相比之下，这两种昆虫在脱落酸（ABA）生物合成突变体aba2-1和aba3-1中产生了相似的转录物特征，ABA在昆虫攻击的植物中负向和正向控制转录物水平。根据转录标记，滨夜蛾幼虫对aba2-1突变体表现较好。本研究揭示了ABA在拟南芥抗虫中的新作用，并鉴定了植物抗草食性的一些重要成分。
Insect attack triggers changes in transcript level in plants that are mediated predominantly by jasmonic acid (JA). The implication of ethylene (ET), salicylic acid (SA), and other signals in this response is less understood and was monitored with a microarray containing insect- and defense-regulated genes. Arabidopsis thaliana mutants coil-1, ein2-1, and sid2-1 impaired in JA, ET, and SA signaling pathways were challenged with the specialist small cabbage white (Pieris rapae) and the generalist Egyptian cotton worm (Spodoptera littoralis). JA was shown to be a major signal controlling the upregulation of defense genes in response to either insect but was found to suppress changes in transcript level only in response to P rapae. Larval growth was affected by the JA-dependent defenses, but S. littoralis gained much more weight on coil-1 than P rapae. ET and SA mutants had an altered transcript profile after S. littoralis herbivory but not after P. rapae herbivory. In contrast, both insects yielded similar transcript signatures in the abscisic acid (ABA)-biosynthetic mutants aba2-1 and aba3-1, and ABA controlled transcript levels both negatively and positively in insect-attacked plants. In accordance with the transcript signature, S. littoralis larvae performed better on aba2-1 mutants. This study reveals a new role for ABA in defense against insects in Arabidopsis and identifies some components important for plant resistance to herbivory.