Harvesting, identification and barrier function of human lung microvascular endothelial cells.
Harvesting, identification and barrier function of human lung microvascular endothelial cells.
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DOI：
10.1016/j.vph.2009.12.009
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发表时间：
2010-05
影响因子：
4
通讯作者：
Black, Stephen M.
中科院分区：
文献类型：
--
作者：
Catravas, John D.;Snead, Connie;Dimitropoulou, Christiana;Chang, Albert S. Y.;Lucas, Rudolf;Verin, Alexander D.;Black, Stephen M.
内皮屏障功能障碍是急性肺损伤（ALI）和急性呼吸窘迫综合征（ARDS）的重要发病机制。尽管明确需要以预防和修复内皮屏障功能障碍为目标的方法，但我们对肺微血管内皮通透性的分子调控的理解仍然不完整。培养的肺微血管内皮细胞是研究肺屏障功能的一个有吸引力的范例。本文介绍了一种人肺微血管内皮细胞（HLMVEC）的收获、鉴定和培养方法。因此获得的HLMVEC作为单层生长，表现出接触抑制，并具有典型的鹅卵石外观。它们表达内皮蛋白，如血管性血友病因子和内皮一氧化氮合酶，并摄取乙酰化LDL。此外，HLMVEC对革兰氏阳性和革兰氏阴性细菌产物、凝血酶、血管内皮生长因子和微管破坏剂的屏障破坏作用的反应是可预测的，并且具有上级敏感性。这些HLMVEC为研究ALI和ARDS的机制提供了一种可替代市售人类细胞的内部衍生物。
Endothelial barrier dysfunction is an important contributor to the pathogenesis of acute lung injury (ALI) and acute respiratory distress syndrome (ARDS). Even though approaches that target the prevention and repair of endothelial barrier dysfunction are clearly needed, our understanding of the molecular regulation of pulmonary microvascular endothelial permeability remains incomplete. Cultured pulmonary microvascular endothelial cells represent an attractive paradigm for the study of barrier function. Here, we describe a method for the harvest, identification and culture of human lung microvascular endothelial cells (HLMVEC). HLMVEC thus obtained, grow as a monolayer, exhibit contact inhibition and have the typical cobblestone appearance. They express endothelial proteins, such as von Willebrand Factor and endothelial nitric oxide synthase and take up acetylated LDL. Furthermore, HLMVEC respond predictably and with superior sensitivity to the barrier disruptive effects of Gram positive and Gram negative bacterial products, thrombin, vascular endothelial growth factor and microtubule disrupting agents. These HLMVEC present an in-house-derived alternative to commercially available human cells for the study of mechanisms contributing to ALI and ARDS.
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DOI：
10.1164/rccm.200702-291oc
发表时间：
2007-10-01
影响因子：
24.7
作者：
Chatterjee, Anuran;Dimitropoulou, Christiana;Catravas, John D.
通讯作者：
Catravas, John D.
DOI：
10.1165/rcmb.2007-0324oc
发表时间：
2008-11-01
影响因子：
6.4
作者：
Antonov, Alexander;Snead, Connie;Catravas, John D.
通讯作者：
Catravas, John D.
影响因子：
5.6
作者：
Birukova, AA;Birilikov, KG;Verin, AD
通讯作者：
Verin, AD
DOI：
10.1152/ajplung.00350.2007
发表时间：
2008-04-01
影响因子：
4.9
作者：
Chatterjee, Anuran;Snead, Connie;Catravas, John D.
通讯作者：
Catravas, John D.
影响因子：
3.7
作者：
ANANTHRAMAN, A;ISRAEL, RH;MAGNUSSEN, CR
通讯作者：
MAGNUSSEN, CR