Expression of cell-cycle-dependent genes in phytohemagglutinin-stimulated human lymphocytes.
Expression of cell-cycle-dependent genes in phytohemagglutinin-stimulated human lymphocytes.
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植物血凝素刺激的人淋巴细胞中细胞周期依赖性基因的表达。
DOI：
10.1073/pnas.82.16.5375
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发表时间：
1985
影响因子：
11.1
通讯作者：
Baserga,R
中科院分区：
文献类型：
--
作者：
Kaczmarek,L;Calabretta,B;Baserga,R
我们研究了植物血凝素（PHA）刺激的人外周血单个核细胞（PBMC）中某些细胞周期依赖基因的表达。所研究的基因先前已被确定为在来自不同物种的其他细胞类型中依赖于细胞周期，并且由不同的有丝分裂原诱导。这些基因之一（2F1）和白细胞介素2受体的基因诱导的PHA，即使在部分耗尽的辅助细胞的淋巴细胞的大小增长，但未能进入S期的文化。其他基因（c-myc、4F1、JE-3和KC-1）仅在PHA刺激的PBMC的完全培养物中被诱导。这些结果证实了在细胞周期进程中可能发生的大小增长和细胞DNA复制之间的分离。此外，这些基因的RNA的可检测水平的出现的时间过程表明，它们可以被用作淋巴细胞从G0期到S期的过渡中的细胞周期进展的标志物。
We have investigated the expression of certain cell-cycle-dependent genes in human peripheral blood mononuclear cells (PBMC) stimulated by phytohemagglutinin (PHA). The genes studied had been previously identified as cell-cycle dependent in other cell types from different species and were induced by different mitogens. One of these genes (2F1) and the gene for the interleukin 2 receptor were induced by PHA even in cultures partially depleted of accessory cells where the lymphocytes grew in size but failed to enter S phase. The other genes (c-myc, 4F1, JE-3, and KC-1) were induced only in complete cultures of PBMC stimulated by PHA. These results confirm the dissociation between growth in size and cell DNA replication that can occur during cell-cycle progression. Moreover, the time course of appearance of detectable levels of RNA for these genes suggests that they may be used as markers of cell-cycle progression in the transition of lymphocytes from G0 to S phase.