Ganoderma lucidum mycelium and spore extracts as natural adjuvants for immunotherapy
Ganoderma lucidum mycelium and spore extracts as natural adjuvants for immunotherapy
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DOI：
10.1089/acm.2005.11.1047
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发表时间：
2005-12-01
影响因子：
2.6
通讯作者：
Chan, GCF
中科院分区：
文献类型：
--
作者：
Chan, WK;Lam, DTW;Chan, GCF
目的：灵芝（GL）是东方社区最常用的中草药之一，超过30%的儿童癌症患者服用GL。GL提取物的免疫调节和抗癌作用已通过体外和体内研究得到证实。然而，没有GL菌丝体提取物（GL-M）和孢子提取物对人体免疫细胞的免疫调节作用之间的比较。树突状细胞（Dendritic cells，DC）是一种专职的抗原提呈细胞，其在DC肿瘤疫苗中的作用已被明确。GL作为人DCs天然佐剂的可能性仍然未知。设计：本研究探讨了GL-M和GL孢子提取物（GL-S）对人外周血单核细胞（PBMC）和单核细胞增殖和Th 1/Th 2细胞因子mRNA表达的差异作用。结果：GL-M可诱导人外周血单个核细胞和单核细胞的增殖，而GL-S则表现出轻微的抑制作用。两种提取物均能刺激Th 1和Th 2细胞因子mRNA的表达，但GL-M对Th 1细胞因子的刺激作用相对较强。与GL-S不同，GL-M在上调CD 40、CD 80和CD 86方面促进DC成熟，并且还减少异硫氰酸荧光素-葡聚糖内吞作用。有趣的是，GL-M处理的DC只有温和的增强淋巴细胞增殖在同种异体混合淋巴细胞culture与轻度增强Th development.Conclusion：这些研究结果提供了证据，GL-M对人类免疫细胞的免疫调节作用，因此可以作为一种天然的佐剂与DC的癌症免疫治疗。
Objectives: Ganoderma lucidum (GL) is one of the most commonly used Chinese herbs in the oriental community, with more than 30% of pediatric cancer patients taking GL. The immunomodulating and anticancer effects exerted by GL extracts have been demonstrated by in vitro and in vivo studies. There was, however, no comparison between the immunomodulating effects of GL mycelium extract (GL-M) and spore extracts on human immune cells. Dendritic cells (DCs) are professional antigen-presenting cells and their role in DC-based tumor vaccine has been well defined. The possibility of GL as natural adjuvant for human DCs remains unknown.Design: This study explored the differential effect of GL-M and GL spore extract (GL-S) on proliferation and Th1/Th2 cytokine mRNA expression of human peripheral blood mononuclear cells (PBMCs) and monocytes. Their effects on the phenotypic and functional maturation of human monocyte-derived DCs were also investigated.Results: GL-M induced the proliferation of PBMCs and monocytes, whereas GL-S showed a mild suppressive effect. Both extracts could stimulate Th1 and Th2 cytokine mRNA expression, but GL-M was a relatively stronger Th1 stimulator. Different from GL-S, GL-M enhanced maturation of DCs in terms of upregulation of CD40, CD80, and CD86, and also reduced fluorescein isothiocyanate-dextran endocytosis. Interestingly, GL-M-treated DCs only modestly enhanced lymphocyte proliferation in allogenic mixed lymphocyte culture with mild enhancement in Th development.Conclusion: These findings provide evidences that GL-M has immunomodulating effects on human immune cells and therefore can be used as a natural adjuvant for cancer immunotherapy with DCs.