Broad-Host-Range Plasmids in Treated Wastewater Effluent and Receiving Streams
Broad-Host-Range Plasmids in Treated Wastewater Effluent and Receiving Streams
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DOI：
10.2134/jeq2010.0228
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发表时间：
2010-11-01
影响因子：
2.4
通讯作者：
Savin, Mary C.
中科院分区：
文献类型：
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作者：
Akiyama, Tatsuya;Asfahl, Kyle L.;Savin, Mary C.
确定了阿肯色州西北部Mud Creek和Spring Creek的两个污水处理厂（WWTP）流出物和流出物接收流中属于不相容（Inc）组印加/C、IncN、IncP和IncW的宽宿主范围（BHR）质粒扩增子的发生。用滤膜上捕获的群落DNA和从Mud Creek分离的抗生素耐药大肠杆菌中提取的质粒DNA进行BHR质粒特异性部分基因序列的聚合酶链反应扩增。IncP质粒扩增产物在两个流的流出物和下游位点中均被检测到，而IncN和IncW质粒扩增产物在Spring Creek的流出物中被检测到，下游但非上游印加/C质粒扩增产物在所有位点中被检测到，包括Spring Creek的大多数样品的上游和Mud Creek的一个样品。一个IncP和两个IncN是唯一的BHR质粒扩增子中发现的85个筛选耐药大肠杆菌分离株，仅在分离株的流出物和下游样品。广宿主范围的质粒经常携带抗生素抗性基因，并且可以促进这些基因的水平转移。虽然在WWTP中检测到BHR质粒，但WWTP并不针对这些遗传元件进行破坏。这项研究表明，BHR质粒是在污水处理厂的流出物，并引入BHR质粒到流。此外，大肠杆菌以外的物种可能是更好的目标作为指示细菌，为未来的研究处理后的污水对环境传播的BHR质粒的影响。
The occurrence of broad-host-range (BHR) plasmid amplicons belonging to incompatibility (Inc) groups IncA/C, IncN, IncP, and IncW in two wastewater treatment plant (WWTP) effluents and effluent receiving streams in Northwest Arkansas, Mud Creek and Spring Creek, was determined. Community DNA captured on filter membranes and plasmid DNA extracted from antibiotic resistant Escherichia colt isolated from Mud Creek was used for polymerase chain reaction amplification of partial gene sequences specific to BHR plasmids. IncP plasmid amplicons were detected in effluent and downstream sites in both streams, while IncN and IncW plasmid amplicons were detected in Spring Creek in effluent and downstream but not upstream IncA/C plasmid amplicons, in contrast, were detected at all sites, including upstream in most samples in Spring Creek and in one sample from Mud Creek. One IncP and two IncN were the only BHR plasmid amplicons found in 85 screened antibiotic-resistant E colt isolates, and were detected only in isolates from effluent and downstream samples. Broad-host-range plasmids frequently carry antibiotic resistance genes and can facilitate horizontal transfer of those genes. While BHR plasmids have been detected in WWTPs, WWTPs do not target these genetic elements for destruction. This study indicates that BHR plasmids are in WWTP effluent and are introducing BHR plasmids into streams. Additionally, species other than E colt may be better targets as indicator bacteria for future studies of the impact of treated effluent on environmental dissemination of BHR plasmids.