FLUORODEOXYURIDINE SYNCHRONIZATION OF BONE-MARROW CULTURES
FLUORODEOXYURIDINE SYNCHRONIZATION OF BONE-MARROW CULTURES
复制标题
DOI：
10.1016/0165-4608(83)90044-4
复制
发表时间：
1983-01-01
影响因子：
--
通讯作者：
GARSON, OM
中科院分区：
文献类型：
--
作者：
WEBBER, LM;GARSON, OM
提出了一种用氟脱氧尿苷 (FdU) 同步骨髓细胞的方法，并与甲氨蝶呤 (MTX) 同步进行比较。 FdU 的优点是不需要洗涤细胞即可释放 DNA 合成模块，被发现对骨髓培养物（来自患有多种血液恶性肿瘤的患者）更有益，因为它通常产生更高的有丝分裂产量，并且对染色体的损害较小。释放带有溴脱氧尿苷 (BrdU) 的 FdU 块后产生的晚期复制带表明，在块释放时处于合成中期的细胞是那些染色体长度增加最多、中期扩散质量改善最多的细胞。因此，由于骨髓细胞比受刺激的淋巴细胞具有更长的细胞周期时间，因此建议在释放块后至少培养 7-8 小时，以获得最佳结果。还发现，为了增加有丝分裂的产量，在添加这些同步剂之前至少需要生长6-8小时。
A method for synchronization of bone marrow cells with fluorodeoxyuridine (FdU) is presented and compared with methotrexate (MTX) synchronization. FdU has the advantage of not requiring cell washing for release of the DNA synthesis block was found to be more beneficial for bone marrow cultures [from patients with a variety of hematological malignancies] because it generally produced a higher mitotic yield and was less damaging to chromosomes. Late-replication banding, produced after releasing the FdU block with bromodeoxyuridine (BrdU), indicated that cells in midsynthesis at the time of the block release were those that showed the most increase in chromosome length and the most improvement in the quality of the metaphase spread. Therefore, because bone marrow cells have a longer cell cycle time than stimulated lymphocytes, a minimum of 7-8 h culture after release of the block is recommended to give optimal results. It was also found that, to increase the yield of mitoses, at least 6-8 h of growth was necessary before the addition of either of these synchronizing agents.