Ultra-thin layer chromatography (UTLC) - A new dimension in thin-layer chromatography
Ultra-thin layer chromatography (UTLC) - A new dimension in thin-layer chromatography
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DOI：
10.1556/jpc.14.2001.4.2
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发表时间：
2001-07-01
影响因子：
1.6
通讯作者：
Schulz, M
中科院分区：
文献类型：
--
作者：
Hauck, HE;Bund, O;Schulz, M
小型化是现代分析方法的总趋势，主要目标是提高灵敏度、大幅缩短分析时间、减少每次分析的耗材数量。在平面色谱中，朝这个方向迈出的第一步是现代高性能薄层色谱 (HPTLC) 的开发。与传统的薄层色谱法相比，这些层的特点是吸附剂材料粒径更小，层厚度略有减小。小型化平面色谱方向的最后一个重要步骤是新的超薄层。与 TLC 和 HPTLC 板上的层相比，这些层不再基于颗粒吸附剂，而是具有基于硅胶基质的整体结构。这意味着不再像 TLC 和 HPTLC 那样存在单独的颗粒。此外，不需要粘合剂来将该层固定在玻璃板上。为了适合色谱用途，这些新型超薄层的整体式硅胶具有细孔和大孔，细毛细管穿透该层。层厚度约为 10 克，明显小于传统 TLC 和 HPTLC 层的厚度。这些新型 UTLC 硅胶板的令人兴奋的特性，特别是它们的选择性和分离效率，通过类固醇、农药和一些染料的分离得到了证明。
Miniaturization is general trend in modern analytical methods, with the main targets of increasing sensitivity, shortening analysis times drastically, and reducing the quantity of consumables per analysis. In planar chromatography a first step in this direction was the development of modern high-performance TLC (HPTLC). Compared with conventional TLC, the layers are characterized by smaller adsorbent material particle sizes and a slight reduction in layer thickness. A major final step in the direction of miniaturized planar chromatography are the new ultra-thin layers. In contrast with those on TLC and HPTLC plates, these layers are no longer based on granular adsorbents, but have a monolithic structure based on a silica gel matrix. This means there are no longer separate particles, as in TLC and HPTLC. Furthermore, no binder is needed to fix the layer on the glass plate. To be suitable for chromatographic purposes, the monolithic silica gel of these new ultra-thin layers has meso- and macro pores, with fine capillaries penetrating the layer. The layer thickness of approximately 10 gm is considerably less than that of conventional TLC and HPTLC layers. The exciting properties of these new UTLC silica gel plates, especially their selectivity and separation efficiency, are demonstrated by separations of steroids, pesticides, and some dyestuffs.