The sidedness of carnitine acetyltransferase and carnitine octanoyltransferase of rat liver endoplasmic reticulum.
The sidedness of carnitine acetyltransferase and carnitine octanoyltransferase of rat liver endoplasmic reticulum.
复制标题
大鼠肝内质网肉碱乙酰转移酶和肉碱辛酰转移酶的侧面性。
DOI：
10.1016/0005-2736(82)90190-0
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发表时间：
1982
期刊：
影响因子：
--
通讯作者：
Bieber,LL
中科院分区：
文献类型：
--
作者：
Valkner,KJ;Bieber,LL
研究了肉毒碱乙酰转移酶和肉毒碱辛酰转移酶在大鼠肝微粒体内外表面的定位。甘露糖-6-磷酸磷酸酶的潜伏期显示微粒体被90-94%密封。所有的辛酰基转移酶都与胞质面相关，而乙酰转移酶分布在内质网膜的胞质面（68-73%）和腔面（27-32%）之间。少量的胰蛋白酶抑制肉毒碱辛酰转移酶同样在密封或渗透性微粒体，但乙酰转移酶的密封微粒体的刺激。大量的胰蛋白酶抑制所有的转移酶活性约60%，除了乙酰转移酶的密封微粒体。其他研究表明，0.1%Triton X-100部分抑制微粒体的肉毒碱辛酰转移酶，但不抑制乙酰转移酶或任何线粒体肉毒碱酰基转移酶。
The location of carnitine acetyltransferase and carnitine octanoyltransferase on the inner and outer surfaces of rat liver microsomes was investigated. Latency of mannose-6-phosphate phosphatase showed that the microsomes were 90–94% sealed. All of the octanoyltransferase is associated with the cytosolic face, while the acetyltransferase is distributed between the cytosolic face (68–73%) and the lumen face (27–32%) of the endoplasmic reticulum membrane. Small amounts of trypsin inhibit the carnitine octanoyltransferase equally in either sealed or permeable microsomes but the acetyltransferase of sealed microsomes is stimulated. Large amounts of trypsin inhibit all transferase activities by about 60%, except for acetyltransferase of sealed microsomes. Other studies show that 0.1% Triton X-100 partially inhibits carnitine octanoyltransferase of microsomes but does not inhibit the acetyltransferase or any of the mitochondrial carnitine acyltransferase.