Comprehensive analysis of gene expression profiles provides insight into the pathogenesis of Crohn's disease.
Comprehensive analysis of gene expression profiles provides insight into the pathogenesis of Crohn's disease.
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DOI：
10.3892/mmr.2018.9267
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发表时间：
2018-09
影响因子：
3.4
通讯作者：
Yang J
中科院分区：
文献类型：
--
作者：
Bo L;Fu H;Yang J
克罗恩病（CD）是一种炎症性肠病，不能通过药物或手术完全治愈。在本研究中，目的是了解CD的潜在机制。从the Gene Expression Omnibus数据库下载了两个CD芯片数据集：GSE36807（13个CD和7个正常样本）和GSE59071（8个CD和11个正常样本）。进行了一系列的生物信息学分析，包括加权基因共表达网络分析以确定稳定模块，以及CD与正常样本之间的差异表达基因（DEGs）分析。筛选GSE36807和GSE59071数据集中的常见基因。随后，选择稳定模块和deg中的重叠基因，使用Cytoscape软件构建蛋白-蛋白相互作用（PPI）网络。对网络中的基因进行富集分析，探索其生物学功能。共鉴定出10个稳定模块和927个基因片段，其中稳定模块和基因片段共有234个基因片段。在去除32个非特征基因后，选择202个基因构建PPI网络。低密度脂蛋白受体（LDLR）、toll样受体2 （TLR2）、脂蛋白脂肪酶（LPL）、叉头盒蛋白M1 （FOXM1）和神经肽Y （NPY）是高度相关的关键节点。途径富集分析表明，LPL在过氧化物酶体增殖物激活受体（PPAR）信号通路中富集。综上所述，PPAR信号通路中的LDLR、TLR2、FOXM1和NPY以及LPL可能在CD的发病机制中发挥重要作用。
Crohn's disease (CD) is a type of inflammatory bowel disease that cannot be fully cured by medication or surgery. In the present study, the aim was to understand the underlying mechanisms of CD. Two CD microarray datasets were downloaded from The Gene Expression Omnibus database: GSE36807 (13 CD and 7 normal samples) and GSE59071 (8 CD and 11 normal samples). A series of bioinformatics analyses were conducted, including weighted gene co-expression network analysis to identify stable modules, and analysis of differentially expressed genes (DEGs) between CD and normal samples. The common DEGs in the GSE36807 and GSE59071 datasets were screened. Subsequently, overlapping genes in the stable modules and the DEGs were selected to construct a protein-protein interaction (PPI) network using Cytoscape software. Enrichment analysis of genes in the network was performed to explore their biological functions. A total of 10 stable modules and 927 DEGs were identified, of which 234 genes were shared in the stable modules and the DEGs. After removal of 32 uncharacterized genes, 202 genes were selected to build the PPI network. Low density lipoprotein receptor (LDLR), toll-like receptor 2 (TLR2), lipoprotein lipase (LPL), forkhead box protein M1 (FOXM1) and neuropeptide Y (NPY) were revealed as key nodes with high degree. Pathway enrichment analysis demonstrated that LPL was enriched in the peroxisome proliferator-activated receptor (PPAR) signaling pathway. In conclusion, LDLR, TLR2, FOXM1 and NPY, as well as LPL in the PPAR signaling pathway may serve critical roles in the pathogenesis of CD.
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影响因子：
9.3
作者：
Herro R;Croft M
通讯作者：
Croft M
WGCNA：用于加权相关网络分析的 R 包。
影响因子：
3
作者：
Langfelder P;Horvath S
通讯作者：
Horvath S
影响因子：
24.5
作者：
Kobayashi, T.;Okamoto, S.;Hibi, T.
通讯作者：
Hibi, T.
影响因子：
3.2
作者：
Frolova, Lenka;Drastich, Pavel;Tlaskalova-Hogenova, Helena
通讯作者：
Tlaskalova-Hogenova, Helena
DOI：
10.1152/ajpgi.00182.2004
发表时间：
2005-03-01
影响因子：
4.5
作者：
Hassani, H;Lucas, G;Ernfors, P
通讯作者：
Ernfors, P