Rate-limiting events in hyperthermic cell killing.
Rate-limiting events in hyperthermic cell killing.
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DOI：
10.2307/3574844
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发表时间：
1978-09
影响因子：
3.4
通讯作者：
J. Landry;N. Marceau
中科院分区：
文献类型：
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作者：
J. Landry;N. Marceau
通过考虑细胞自由能$（\Delta G^{+}）$对温度的依赖性，从热力学角度分析了HeLa细胞在41至55°C温度范围内和2至300分钟处理时间范围内的灭活率。在该温度范围内，增殖能力的损失表现出复杂的温度依赖性，其特征在于熵和焓值随着温度的升高而逐渐降低。这种复杂的热诱导细胞杀伤过程被认为是一系列反应的结果，其中每一个都在一定的温度范围内交替限制速率。从该动力学方案推导出数学模型，并且在HeLa细胞的情况下，使用最小二乘搜索参数程序（如应用于推导出的存活回归函数）证明了三个这样的顺序反应足以解释在41至55°C范围内获得的所有实验数据点。提出的模型也…
The inactivation rate of HeLa cells for temperatures ranging from 41 to 55°C and treatment durations varying from 2 to 300 min was analyzed in thermodynamic terms by considering the dependence of cell free energy $(\Delta G^{+})$ on temperature. Within this temperature range the loss of proliferative capacity exhibits a complex temperature dependence which is characterized by entropy and enthalpy values that gradually decrease as temperature increases. This complex process of heat-induced cell killing was postulated to be the result of a series of reactions, each of them being alternatively rate limiting within a certain temperature range. From this kinetic scheme a mathematical model was derived and, in the case of HeLa cells, the use of a least-squares search parameter procedure (as applied to the derived survival regression function) demonstrated that three such sequential reactions were sufficient to explain all experimental data points obtained within the 41 to 55°C range. The proposed model was also...