Clinical magnetic resonance imaging of pancreatic islet grafts after iron nanoparticle labeling
Clinical magnetic resonance imaging of pancreatic islet grafts after iron nanoparticle labeling
复制标题
DOI：
10.1111/j.1600-6143.2007.02120.x
复制
发表时间：
2008-03-01
影响因子：
8.8
通讯作者：
Berney, T.
中科院分区：
文献类型：
--
作者：
Toso, C.;Valle, J. -P.;Berney, T.
细胞移植后迫切需要无创评估工具。本研究探讨磁共振成像（MRI）策略是否可以临床应用于胰岛移植。七种人胰岛制剂的最纯部分用超顺磁性氧化铁颗粒（SPIO，280 μg/mL）标记，并移植到四名 1 型糖尿病患者体内。 MRI 研究 (T2*) 在移植前和移植后的不同时间点进行。标记胰岛的活力以及体外和体内功能与对照胰岛相似。所有患者移植后均可停用胰岛素。第一个患者的基线肝脏 MRI 上有弥漫性低信号图像，典型的自发性高铁含量，并且无法观察到移植相关的改变。其他三名患者在移植前图像上具有正常强度，并且在移植后可以将载铁胰岛识别为肝脏内的低信号点。其中之一，i.v.由于弥漫性低信号肝脏背景，铁治疗阻止了随后斑点的可视化。总而言之，本研究证明了基于 MRI 的胰岛移植监测在临床实践中的可行性和安全性。铁过载（自发的或诱导的）是该技术的主要障碍。
There is a crucial need for noninvasive assessment tools after cell transplantation. This study investigates whether a magnetic resonance imaging (MRI) strategy could be clinically applied to islet transplantation. The purest fractions of seven human islet preparations were labeled with superparamagnetic iron oxide particles (SPIO, 280 mu g/mL) and transplanted into four patients with type 1 diabetes. MRI studies (T2*) were performed prior to and at various time points after transplantation. Viability and in vitro and in vivo functions of labeled islets were similar to those of control islets. All patients could stop insulin after transplantation. The first patient had diffuse hypointense images on her baseline liver MRI, typical for spontaneous high iron content, and transplant-related modifications could not be observed. The other three patients had normal intensity on pretransplant images, and iron-loaded islets could be identified after transplantation as hypointense spots within the liver. In one of them, i.v. iron therapy prevented subsequent visualization of the spots because of diffuse hypointense liver background. Altogether, this study demonstrates the feasibility and safety of MRI-based islet graft monitoring in clinical practice. Iron overload (spontaneous or induced) represents the major obstacle to the technique.