Cyclic mechanical strain regulates the development of engineered smooth muscle tissue
Cyclic mechanical strain regulates the development of engineered smooth muscle tissue
复制标题
DOI：
10.1038/13671
复制
发表时间：
1999-10-01
影响因子：
46.9
通讯作者：
Mooney, DJ
中科院分区：
文献类型：
--
作者：
Kim, BS;Nikolovski, J;Mooney, DJ
我们发现，机械刺激和聚合物支架的适当组合可以提高工程组织的力学性能。由细胞和聚合物支架构建的组织的力学性能明显低于它们所替代的天然组织。我们假设，对工程组织施加机械刺激会改变它们的机械性能。将平滑肌组织工程化于两种不同的聚合物支架上，并承受循环机械应变。短期应用菌株可增加平滑肌细胞(SMC)的增殖以及胶原和弹性蛋白的表达，但只有当SMC附着在特定支架上时才会增加。长期应用周期性应变可上调弹性蛋白和胶原基因的表达，并导致组织中组织化程度增加。这导致组织的机械性能增加了一个数量级以上。
We show that the appropriate combinations of mechanical stimuli and polymeric scaffolds can enhance the mechanical properties of engineered tissues. The mechanical properties of tissues engineered from cells and polymer scaffolds are significantly lower than the native tissues they replace. We hypothesized that application of mechanical stimuli to engineered tissues would alter their mechanical properties. Smooth muscle tissue was engineered on two different polymeric scaffolds and subjected to cyclic mechanical strain. Short-term application of strain increased proliferation of smooth muscle cells (SMCs) and expression of collagen and elastin, but only when SMCs were adherent to specific scaffolds. Long-term application of cyclic strain upregulated elastin and collagen gene expression and led to increased organization in tissues. This resulted in more than an order of magnitude increase in the mechanical properties of the tissues.