Effects of a High Fat Diet and Voluntary Wheel Running Exercise on Cidea and Cidec Expression in Liver and Adipose Tissue of Mice.
Effects of a High Fat Diet and Voluntary Wheel Running Exercise on Cidea and Cidec Expression in Liver and Adipose Tissue of Mice.
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DOI：
10.1371/journal.pone.0130259
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Puri V
中科院分区：
文献类型：
--
作者：
Reynolds TH 4th;Banerjee S;Sharma VM;Donohue J;Couldwell S;Sosinsky A;Frulla A;Robinson A;Puri V
Cidea和Cidec在调节肝脏和脂肪组织中甘油三酯储存中起重要作用。目前尚不清楚Cidea和Cidec基因是否对高脂饮食（HFD）或运动训练有反应，这两种干预措施会改变脂质储存。本研究的目的是确定HFD和自愿轮跑（WR）对小鼠脂肪组织和肝脏中Cidea和Cidec mRNA和蛋白表达的影响。HFD促进脂肪组织和肝脏中Cidea和Cidec mRNA水平的显著增加。WR阻止了脂肪组织和肝脏中响应HFD的Cidea和Cidec mRNA的增加。与Cidea mRNA的变化类似，脂肪组织中的Cidea蛋白水平在HFD的反应中显著增加，这一过程再次被WR阻止。然而，在脂肪组织中，Cidec mRNA的变化并不对应于Cidec蛋白水平的变化，因为HFD降低了Cidec蛋白丰度。有趣的是，在脂肪组织中，Cidea蛋白表达与体重（R= 0.725）、附睾脂肪组织（EWAT）质量（R= 0.475）和胰岛素抵抗（R= 0.706）显著相关，而Cidec蛋白表达与体重（R=-0.787）、EWAT质量（R=-0.706）和胰岛素抵抗（R=-0.679）负相关。与脂肪组织相似，肝脏中的Cidea蛋白表达与体重（R= 0.660）、EWAT质量（R= 0.468）和胰岛素抵抗（R= 0.599）显著相关;然而，与脂肪组织不同，肝脏中的Cidec蛋白水平与体重或EWAT质量无关，仅与胰岛素抵抗中度相关（R=-0.422，P=0.051）。总的来说，我们的研究结果表明，Cidea与肥胖和胰岛素抵抗高度相关，而Cidec与胰岛素敏感性相关。本研究提示锡德蛋白可能在肥胖、肝脂肪变性以及2型糖尿病的发病机制中起重要作用。
Cidea and Cidec play an important role in regulating triglyceride storage in liver and adipose tissue. It is not known if the Cidea and Cidec genes respond to a high fat diet (HFD) or exercise training, two interventions that alter lipid storage. The purpose of the present study was to determine the effect of a HFD and voluntary wheel running (WR) on Cidea and Cidec mRNA and protein expression in adipose tissue and liver of mice. A HFD promoted a significant increase in Cidea and Cidec mRNA levels in adipose tissue and liver. The increase in Cidea and Cidec mRNAs in adipose tissue and liver in response to a HFD was prevented by WR. Similar to the changes in Cidea mRNA, Cidea protein levels in adipose tissue significantly increased in response to a HFD, a process that was, again, prevented by WR. However, in adipose tissue the changes in Cidec mRNA did not correspond to the changes in Cidec protein levels, as a HFD decreased Cidec protein abundance. Interestingly, in adipose tissue Cidea protein expression was significantly related to body weight (R=.725), epididymal adipose tissue (EWAT) mass (R=.475) and insulin resistance (R=.706), whereas Cidec protein expression was inversely related to body weight (R=-.787), EWAT mass (R=-.706), and insulin resistance (R=-.679). Similar to adipose tissue, Cidea protein expression in liver was significantly related to body weight (R=.660), EWAT mass (R=.468), and insulin resistance (R=.599); however, unlike adipose tissue, Cidec protein levels in liver were not related to body weight or EWAT mass and only moderately associated with insulin resistance (R=-.422, P=0.051). Overall, our findings indicate that Cidea is highly associated with adiposity and insulin resistance, whereas Cidec is related to insulin sensitivity. The present study suggests that Cide proteins might play an important functional role in the development of obesity, hepatic steatosis, as well as the pathogenesis of type 2 diabetes.