Human glioblastoma cell lines have neuropeptide receptors for bombesin/gastrin-releasing peptide.
Human glioblastoma cell lines have neuropeptide receptors for bombesin/gastrin-releasing peptide.
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人胶质母细胞瘤细胞系具有铃蟾肽/胃泌素释放肽的神经肽受体。
DOI：
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发表时间：
1989
影响因子：
3.1
通讯作者：
R. Kris
中科院分区：
文献类型：
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作者：
T. Moody;S. Mahmoud;J. Staley;L. Naldini;D. Cirillo;V. South;S. Felder;R. Kris
在人脑胶质母细胞瘤细胞系中鉴定了蛙皮素/胃泌素释放肽受体。[125I]与这些细胞系高亲和力结合的胃泌素释放肽或([125I]Tyr4)蛙皮素。与U-118细胞的结合具有时间依赖性、可逆性和特异性。([125I]Tyr4)与单类位点(Bmax=30,000/cell)高亲和力(Kd=1.6 nm)结合的蛙素。蛙皮素或胃泌素释放肽的C末端是高亲和力结合所必需的。蛙皮素或胃泌素释放肽以剂量依赖的方式升高细胞内钙离子水平。由于胃泌素释放肽，而不是胃泌素释放肽，增加了细胞内钙离子水平，所以GRP的C端而不是N端是生物活性所必需的。这些数据表明，在人脑胶质母细胞瘤细胞系中存在具有生物活性的蛙皮素受体。
Bombesin/gastrin-releasing peptide receptors were characterized in human glioblastoma cell lines. [125I]Gastrin-releasing peptide or ([125I]Tyr4)bombesin bound with high affinity to these cell lines. Binding to cell line U-118 was time dependent, reversible, and specific. ([125I]Tyr4)Bombesin bound with high affinity (Kd = 1.6 nM) to a single class of sites (Bmax = 30,000/cell). The C-terminal of bombesin- or gastrin-releasing peptide was essential for high-affinity binding. Bombesin- or gastrin-releasing peptide elevated the cytosolic Ca2+ levels in a dose-dependent manner. Because gastrin-releasing peptide, but not gastrin-releasing peptide, increased the cytosolic Ca2+ levels, the C-terminal but not the N-terminal of GRP is essential for biological activity. These data indicate that biologically active bombesin receptors are present in human glioblastoma cell lines.