Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans.
Gas Chromatography/Mass Spectrometry-Based Metabolomic Profiling Reveals Alterations in Mouse Plasma and Liver in Response to Fava Beans.
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基于气相色谱/质谱的代谢组学分析揭示了蚕豆对小鼠血浆和肝脏的影响。
DOI：
10.1371/journal.pone.0151103
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发表时间：
2016
期刊：
影响因子：
3.7
通讯作者：
Cai W
中科院分区：
文献类型：
--
作者：
Xiao M;Du G;Zhong G;Yan D;Zeng H;Cai W
蚕豆病是一种危及生命的溶血性贫血，由红细胞葡萄糖6-磷酸脱氢酶（G6 PD）活性低的易感个体摄入蚕豆引起。然而，很少有人知道在G6 PD正常和缺乏状态下摄入蚕豆后血浆和肝脏中的代谢组学变化。在这项研究中，气相色谱/质谱法被用来分析血浆和肝脏的代谢变化的影响蚕豆C3 H-和G6 PD-缺陷（G6 PDx）小鼠，并找到潜在的生物标志物和代谢变化与蚕豆。我们的研究结果表明，蚕豆诱导C3 H和G6 PDx小鼠的氧化应激。值得注意的是，在C3 H和G6 PDx小鼠的对照组和蚕豆处理组之间的血浆和肝脏中观察到代谢组学差异。血浆中7种和21种代谢物的水平分别在C3 H对照（C3 H-C）和C3 H蚕豆处理（C3 H-FB）小鼠与G6 PDx对照（G6 PDx-C）和G6 PDx蚕豆处理（G6 PDx-FB）小鼠之间显示出显著差异。同样，肝脏中7种和25种代谢物的水平分别在C3 H和C3 H-FB之间以及G6 PDx和G6 PDx-FB之间显示出显著差异。C3 H-FB和G6 PDx-FB小鼠血浆中的油酸、亚油酸和肌酐水平均显著升高。在肝脏中，在G6 PDx-FB小鼠中观察到比C3 H-FB小鼠更多的代谢改变，并且涉及糖、脂肪酸、氨基酸、胆固醇生物合成、尿素循环和核苷酸代谢途径。这些研究结果表明，油酸，亚油酸和肌酐可能是C3 H和G6 PDx小鼠对蚕豆反应的潜在生物标志物，因此油酸和亚油酸可能参与蚕豆诱导的氧化应激。这项研究表明，小鼠中的G6 PD活性可以影响它们对蚕豆的代谢途径。
Favism is a life-threatening hemolytic anemia resulting from the intake of fava beans by susceptible individuals with low erythrocytic glucose 6-phosphate dehydrogenase (G6PD) activity. However, little is known about the metabolomic changes in plasma and liver after the intake of fava beans in G6PD normal and deficient states. In this study, gas chromatography/mass spectrometry was used to analyze the plasma and liver metabolic alterations underlying the effects of fava beans in C3H- and G6PD-deficient (G6PDx) mice, and to find potential biomarkers and metabolic changes associated with favism. Our results showed that fava beans induced oxidative stress in both C3H and G6PDx mice. Significantly, metabolomic differences were observed in plasma and liver between the control and fava bean treated groups of both C3H and G6PDx mice. The levels of 7 and 21 metabolites in plasma showed significant differences between C3H-control (C3H-C)- and C3H fava beans-treated (C3H-FB) mice, and G6PDx-control (G6PDx-C)- and G6PDx fava beans-treated (G6PDx-FB) mice, respectively. Similarly, the levels of 7 and 25 metabolites in the liver showed significant differences between C3H and C3H-FB, and G6PDx and G6PDx-FB, respectively. The levels of oleic acid, linoleic acid, and creatinine were significantly increased in the plasma of both C3H-FB and G6PDx-FB mice. In the liver, more metabolic alterations were observed in G6PDx-FB mice than in C3H-FB mice, and were involved in a sugar, fatty acids, amino acids, cholesterol biosynthesis, the urea cycle, and the nucleotide metabolic pathway. These findings suggest that oleic acid, linoleic acid, and creatinine may be potential biomarkers of the response to fava beans in C3H and G6PDx mice and therefore that oleic acid and linoleic acid may be involved in oxidative stress induced by fava beans. This study demonstrates that G6PD activity in mice can affect their metabolic pathways in response to fava beans.