Photodynamically induced alteration of cornea endothelial cell function.
Photodynamically induced alteration of cornea endothelial cell function.
复制标题
光动力诱导角膜内皮细胞功能的改变。
DOI：
--
复制
发表时间：
1979
影响因子：
4.4
通讯作者：
K. Green
中科院分区：
文献类型：
--
作者：
D. S. Hull;E. C. Strickland;K. Green
在角膜内皮显微镜下用不同浓度的虎红灌注角膜内皮细胞。用浓度为10（-6）M至10（-5）M的虎红灌注并暴露于光下0.5至5 min的角膜肿胀速率随着虎红浓度的增加和光暴露时间的增加而更快。用类似浓度的虎红灌注但不暴露于光下的角膜没有肿胀。将虎红与100 μ g/ml超氧化物歧化酶联合给药不会减少暴露于光后的角膜肿胀，表明光动力诱导的内皮虎红灌注液消除了角膜暴露于光后的角膜肿胀。这表明，内皮细胞的光动力效应是继发于细胞功能的改变，从过氧化氢的超氧化物歧化酶催化的超氧化物自由基的歧化反应过程中产生的。
Corneal endothelial cells were perfused in the specular microscope with varying concentrations of rose bengal. Corneas perfused with rose bengal in concentrations of 10(-6)M to 10(-5)M and exposed to light for periods of 0.5 to 5 min swelled at rates which were more rapid with both increasing concentration of rose bengal and increasing duration of light exposure. Corneas perfused with similar concentrations of rose bengal but not exposed to light did not swell. Combining rose bengal with 100 micrograms/ml superoxide dismutase did not reduce the corneal swelling following exposure to light, indicating that the photodynamically induced endothelial bengal perfusing solution eliminated corneal swelling following exposure of corneas to light. This indicates that the photodynamic effect of endothelium is secondary to cell functional alterations from the hydrogen peroxide produced during the dismutation reaction of superoxide free radical which is catalyzed by superoxide dismutase.