GhCFE1A, a dynamic linker between the ER network and actin cytoskeleton, plays an important role in cotton fibre cell initiation and elongation
GhCFE1A, a dynamic linker between the ER network and actin cytoskeleton, plays an important role in cotton fibre cell initiation and elongation
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GhCFE1A 是 ER 网络和肌动蛋白细胞骨架之间的动态连接子，在棉纤维细胞的起始和伸长中发挥重要作用
DOI：
10.1093/jxb/eru530
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发表时间：
2015-04-01
影响因子：
6.9
通讯作者：
Guo, Wangzhen
中科院分区：
文献类型：
--
作者：
Lv, Fenni;Wang, Haihai;Guo, Wangzhen
纤维细胞的发生和伸长是棉纤维发育的关键。然而，鲜为人知的是，在纤维细胞发育过程中的起始和伸长的调节。在这里，一种新的蛋白质GhCFE 1A的调节作用被发现。GhCFE 1A在纤维发育过程中的起始和快速伸长阶段优先表达;此外，GhCFE 1A在无毛棉突变体中的纤维起始阶段的表达比在纤维承载TM-1野生型中高得多。重要的是，棉花中GhCFE 1A的过表达不仅延迟了纤维细胞的伸长，而且还显著降低了皮棉和绒毛纤维起始物和茎毛状体的密度。酵母双杂交实验表明GhCFE 1A与多种肌动蛋白相互作用，共沉淀实验进一步证实了GhCFE 1A与肌动蛋白的相互作用。有趣的是，亚细胞定位分析表明，GhCFE 1A驻留在皮质内质网（ER）网络和共定位与肌动蛋白电缆。此外，与野生型对照相比，在快速伸长阶段GhCFE 1A过表达纤维中F-肌动蛋白丝的密度被证明是减少的。综上所述，这些结果表明，GhCFE 1A可能作为肌动蛋白细胞骨架和ER网络之间的动态连接器，并在棉纤维发育过程中纤维细胞的起始和伸长中起着重要作用。
Fibre cell initiation and elongation is critical for cotton fibre development. However, little is known about the regulation of initiation and elongation during fibre cell development. Here, the regulatory role of a novel protein GhCFE1A was uncovered. GhCFE1A is preferentially expressed at initiation and rapid elongation stages during fibre development; in addition, much higher expression of GhCFE1A was detected at the fibre initiation stage in fibreless cotton mutants than in the fibre-bearing TM-1 wild-type. Importantly, overexpression of GhCFE1A in cotton not only delayed fibre cell elongation but also significantly reduced the density of lint and fuzz fibre initials and stem trichomes. Yeast two-hybrid assay showed that GhCFE1A interacted with several actin proteins, and the interaction was further confirmed by co-sedimentation assay. Interestingly, a subcellular localization assay showed that GhCFE1A resided on the cortical endoplasmic reticulum (ER) network and co-localized with actin cables. Moreover, the density of F-actin filaments was shown to be reduced in GhCFE1A-overexpressing fibres at the rapid elongation stage compared with the wild-type control. Taken together, the results demonstrate that GhCFE1A probably functions as a dynamic linker between the actin cytoskeleton and the ER network, and plays an important role in fibre cell initiation and elongation during cotton fibre development.