Lipoxin A4 and benzo-lipoxin A4 attenuate experimental renal fibrosis
Lipoxin A4 and benzo-lipoxin A4 attenuate experimental renal fibrosis
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DOI：
10.1096/fj.11-185017
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发表时间：
2011-09-01
期刊：
影响因子：
4.8
通讯作者：
Godson, Catherine
中科院分区：
文献类型：
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作者：
Boergeson, Emma;Docherty, Neil G.;Godson, Catherine
未解决的炎症是纤维化和器官衰竭发展的基础。在此，我们研究了在早期肾纤维化、单侧输尿管梗阻（UUO）模型中，前分解类花生酸脂氧素A（4）（LXA（4））及其合成类似物苯并-LXA（4）对纤维化和炎症反应的特异性调节潜力。雄性Wistar大鼠（Animalia，Chordata，Rattus norvegicus）在手术前15分钟静脉注射溶剂（0.1%乙醇）、LXA（4）（45 μ g/250-g大鼠）或苯并-LXA（4）（15 μ g/250-g大鼠），并在结扎后3天处死。使用假手术的操作肾脏作为对照，分析肾脏基因和蛋白质表达、胶原沉积、巨噬细胞浸润和细胞凋亡。脂氧素（LXs）可减少胶原沉积和肾细胞凋亡（P
Unresolved inflammation underlies the development of fibrosis and organ failure. Here, we investigate the potential of the proresolving eicosanoid lipoxinA(4) (LXA(4)) and its synthetic analog benzo-LXA(4) to prophylactically modulate fibrotic and inflammatory responses in a model of early renal fibrosis, unilateral ureteric obstruction (UUO). Male Wistar rats (Animalia, Chordata, Rattus norvegicus) were injected intravenously with vehicle (0.1% ethanol), LXA(4) (45 mu g/250-g rat), or benzo-LXA(4) (15 mu g/250-g rat) 15 min prior to surgery and sacrificed 3 d postligation. Renal gene and protein expression, collagen deposition, macrophage infiltration, and apoptosis were analyzed using manipulated kidneys from sham operations as control. Lipoxins (LXs) attenuated collagen deposition and renal apoptosis (P