Astrocyte secreted proteins selectively increase hippocampal GABAergic axon length, branching, and synaptogenesis.
Astrocyte secreted proteins selectively increase hippocampal GABAergic axon length, branching, and synaptogenesis.
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DOI：
10.1016/j.mcn.2009.10.004
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发表时间：
2010-01
影响因子：
3.5
通讯作者：
Balice-Gordon, Rita J.
中科院分区：
文献类型：
--
作者：
Hughes, Ethan G.;Elmariah, Sarina B.;Balice-Gordon, Rita J.
星形胶质细胞调节中枢神经系统中GABA能突触的形成和功能，但星形胶质细胞是否调节GABA能突触发生尚不清楚。我们证明，条件培养基星形胶质细胞，而不是其他细胞，增强GABA能，但不animatergic轴突长度和分支，并增加突触前活动GABA能突触的数量和密度在分离的海马文化。候选机制和因素，如活动，神经营养因子和胆固醇被排除为介导这些影响。虽然星形胶质细胞分泌的血小板反应蛋白是增加海马神经元突触发生所必需的，但它们不介导星形胶质细胞对GABA能突触发生的作用。我们表明，在星形胶质细胞条件培养基中选择性影响GABA能神经元的因素是蛋白质。总之，我们的研究结果表明，星形胶质细胞通过不同的机制增加谷氨酸能和GABA能突触发生，并释放一种或多种具有增加GABA能轴突长度、分支和突触发生的新功能的蛋白质。
Astrocytes modulate the formation and function of glutamatergic synapses in the CNS, but whether astrocytes modulate GABAergic synaptogenesis is unknown. We demonstrate that media conditioned by astrocytes, but not other cells, enhanced GABAergic but not glutamatergic axon length and branching, and increased the number and density of presynaptically active GABAergic synapses in dissociated hippocampal cultures. Candidate mechanisms and factors, such as activity, neurotrophins, and cholesterol were excluded as mediating these effects. While thrombospondins secreted by astrocytes are necessary and sufficient to increase hippocampal glutamatergic synaptogenesis, they do not mediate astrocyte effects on GABAergic synaptogenesis. We show that the factors in astrocyte conditioned media that selectively affect GABAergic neurons are proteins. Taken together, our results show that astrocytes increase glutamatergic and GABAergic synaptogenesis via different mechanisms and release one or more proteins with the novel functions of increasing GABAergic axon length, branching and synaptogenesis.
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影响因子：
5.3
作者：
Elmariah, SB;Crumling, MA;Balice-Gordon, RJ
通讯作者：
Balice-Gordon, RJ
影响因子：
5.5
作者：
Nägler, K;Mauch, DH;Pfrieger, FW
通讯作者：
Pfrieger, FW
影响因子：
5.3
作者：
Kilman, V;van Rossum, MCW;Turrigiano, GG
通讯作者：
Turrigiano, GG
影响因子：
5.3
作者：
Martens, Henrik;Weston, Matthew C.;Haertig, Wolfgang
通讯作者：
Haertig, Wolfgang
影响因子：
5.3
作者：
Jiao, Yuanyuan;Zhang, Chunzhao;Sun, Qian-Quan
通讯作者：
Sun, Qian-Quan