Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid supports a grapevine origin for hop stunt disease
Phylogenetic analysis of hop and grapevine isolates of hop stunt viroid supports a grapevine origin for hop stunt disease
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DOI：
10.1023/a:1008182302704
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发表时间：
2001-01-01
期刊：
影响因子：
1.6
通讯作者：
Ohshima, K
中科院分区：
文献类型：
--
作者：
Sano, T;Mimura, R;Ohshima, K
我们检测了从日本东北地区啤酒花花园中采集的9株类啤酒花病毒（HSVd）的序列变异性，日本东北地区是世界上唯一的啤酒花病流行地区。在9株HSVd-hop分离株中检测到6个不同的一致性和单一致性序列以及12个序列变异，说明HSVd-hop序列存在显著的变异。将新的HSVd-hop序列与先前从hop和其他物种中分离的44个HSVd变体进行邻居连接分析。从日本东北地区种植的啤酒花中恢复的所有HSVd-hop序列以及该型分离物和两个韩国分离物与通常从葡萄藤中恢复的HSVd-g亚型1形成一个集群。这种HSVd-hop和-grapevine分离物之间的密切关系有力地支持了酒花矮缩病的葡萄起源。
We have examined sequence variability among nine isolates of hop stunt viroid (HSVd) collected from hop gardens in Tohoku district in Japan, the only area in the world where hop stunt disease is endemic. Six different consensus and one-consensus sequences as well as 12 sequence variants were detected in the nine HSVd-hop isolates, which suggested the sequence of HSVd-hop was remarkably variable. A neighbor-joining analysis was carried out on the new HSVd-hop sequences together with 44 previously described variants of HSVd isolated from hop and other species. All the HSVd-hop sequences recovered from hops cultivated in the Tohoku district of Japan as well as the type isolate and two Korean isolates form a cluster with the HSVd-g subtype 1 commonly recovered from grapevine. This close relationship between HSVd-hop and -grapevine isolates strongly supports the grapevine origin for hop stunt disease.