Identification and isozyme characterisation of Meloidogyne spp. infecting horticultural and agronomic crops, and weed plants in Florida
Identification and isozyme characterisation of Meloidogyne spp. infecting horticultural and agronomic crops, and weed plants in Florida
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DOI：
10.1163/156854108785787253
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发表时间：
2008-01-01
期刊：
影响因子：
1.2
通讯作者：
Dickson, Donald W.
中科院分区：
文献类型：
--
作者：
Brito, Janete A.;Kaur, Ramandeep;Dickson, Donald W.
共327根样品收集园艺和农艺作物，杂草与这些作物在佛罗里达作物生产区，进行了检查。同工酶表型，酯酶（EST）和苹果酸脱氢酶（MDH）的表型被用来鉴定和确定存在于这些样品中的根结线虫的种类。使用聚丙烯酰胺凝胶电泳对每个样本中至少26只雌性进行检查。共发现16条主要酶带，对应12种表型。种特异性EST表型始终与每个根结线虫属。鉴定出花生根结线虫Meloidogyne arenaria，M. florescence sis，M. graminicola、草间霉M.哈普拉，M。incognita，M. javanica，M. mayaguensis和M. partityla。发现两种尚未描述的独特EST表型与三种未鉴定的线虫种群相关。MDH酶活性谱带有5条，表现型有4种。在本研究期间，确定了M. florescence sis和M. mayaguensis，两个最近报道的根结线虫属（Meloidogyne spp.）在佛罗里达。新发现的寄主植物种类。floracolus sp.，茄（Solanum melongena）和一点红（Emilia sonchifolia）的种子，以及一点红（M.抗隐名病桃砧木‘Flordaguard’。新寄主M. mayaguensis为番木瓜（Carica papaya）、辣椒（Capsicum annuum var. Longum，以及六种杂草植物，包括匍匐茎，Fatoua villosa，Panicum sp.，一品红、美洲茄以及一种未鉴定的杂草。据我们所知，佛罗里达是北美唯一一个M. florescence sis和M.发现了马亚古虫
A total of 327 root samples collected from horticultural and agronomic crops, and weeds associated with these crops in Florida crop production regions, were examined. Isozyme phenotypes, esterase (EST) and malate dehydrogenase (MDH) phenotypes were used to characterise and identify the species of Meloidogyne present in these samples. At least 26 females from each sample were examined using polyacrylamide gel electrophoresis. Sixteen major bands of EST activity were found, corresponding to 12 phenotypes. A species-specific EST phenotype was consistently associated with each of the Meloidogyne spp. identified: Meloidogyne arenaria, M. floridensis, M. graminicola, M. hapla, M. incognita, M. javanica, M. mayaguensis and M. partityla. Two unique EST phenotypes, which have not been described, were found associated with three unidentified nematode populations. Five bands of MDH activity and four phenotypes were also found among the populations. During this study, new host records were determined for M. floridensis and M. mayaguensis, two recently reported Meloidogyne spp. in Florida. Plant species found as new hosts of M. floridensis were Cucumis sativus, Phaseolus sp., Solanum melongena and one weed species, Emilia sonchifolia, and the M. incognita-resistant peach rootstock 'Flordaguard'. New hosts for M. mayaguensis were Carica papaya, Capsicum annuum var. Longum, and six weed plants, including Eclipta prostrata, Fatoua villosa, Panicum sp., Poinsettia cyathophora, Solanum americanum and also one unidentified weed species belonging to the family Acanthaceae. To our knowledge, Florida is the only geographic area of North America where M. floridensis and M. mayaguensis have been detected.