Ultrasmall Nanopipette: Toward Continuous Monitoring of Redox Metabolism at Subcellular Level
Ultrasmall Nanopipette: Toward Continuous Monitoring of Redox Metabolism at Subcellular Level
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超小型纳米移液器：在亚细胞水平上连续监测氧化还原代谢
DOI：
10.1002/ange.201808537
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发表时间：
2018
期刊：
影响因子：
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通讯作者：
Prof. Hong‐Yuan Chen
中科院分区：
文献类型：
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作者：
Juan Song;Dr. Cong‐Hui Xu;Shi‐Zhen Huang;Wen Lei;Yi‐Fan Ruan;Hai‐Jie Lu;Dr. Wei Zhao;Prof. Dr. Jing‐Juan Xu;Prof. Hong‐Yuan Chen
基于离子电流整流（ICR）的纳米移液管允许精确监测单个活细胞中的细胞行为。在此，我们提出了一种30 nm纳米移液管功能化与G-四链体DNA酶作为一个有效的仿生识别器的ROS产生在亚细胞水平上通过改变电流-电压关系。 利用超小吸头的优势，纳米移液管可以反复渗透到单个活细胞中或长时间保持测量，而不会影响细胞功能。结合精确的纳米定位系统，以高空间分辨率确定响应于细胞炎症的线粒体中ROS的产生。同时，持续监测不同细胞系在药物诱导的氧化应激下有氧代谢的变化。我们相信，ICR纳米移液管可以开发为通过活细胞中的电化学成像研究细胞活动的强大方法。
Ionic current rectification (ICR) based nanopipettes allow accurate monitoring of cellular behavior in single living cells. Herein, we proposed a 30 nm nanopipette functionalized with G‐quadruplex DNAzyme as an efficient biomimetic recognizer for ROS generation at subcellular level via the changes of current–voltage relationship. Taking advantages of the ultra‐small tip, the nanopipette could penetrate into a single living cell repeatedly or keep measuring for a long time without compromising the cellular functions. Coupled with precision nanopositioning system, generation of ROS in mitochondria in response to cell inflammation was determined with high spatial resolution. Meanwhile, the changes of aerobic metabolism in different cell lines under drug‐induced oxidative stress were monitored continuously. We believe that the ICR‐nanopipette could be developed as a powerful approach for the study of cellular activities via electrochemical imaging in living cells.