Identification and characterization of versican/PG-M aggregates in cartilage
Identification and characterization of versican/PG-M aggregates in cartilage
复制标题
DOI：
10.1074/jbc.m510330200
复制
发表时间：
2006-06-30
影响因子：
4.8
通讯作者：
Watanabe, Hideto
中科院分区：
文献类型：
--
作者：
Matsumoto, Kazu;Kamiya, Nobuhiro;Watanabe, Hideto
Versican/PG-M是细胞外基质的大硫酸软骨素蛋白聚糖，具有与聚集蛋白聚糖共同的结构域结构，并且以低水平存在于软骨中。在这里，我们的特点是软骨多功能蛋白聚糖在发展和增长。免疫组化结果显示，versican主要定位在关节面的interterritorial带在2周的小鼠，而聚集蛋白聚糖是在细胞周围区的prehypertrophic和肥大细胞的生长板。虽然它的转录水平在生长过程中迅速下降，但多功能蛋白聚糖仍然存在于关节软骨中。正常关节软骨和软骨基质缺乏症（cartilage matrix deficiency，CMD）/CMD小鼠的无聚集蛋白聚糖软骨的生化分析显示，多功能蛋白聚糖作为连接蛋白和透明质酸的蛋白聚糖聚集体存在。软骨素酶ABC消化的多能蛋白聚糖的硫酸软骨素链含有71%的非硫酸化和28%的4-硫酸化不饱和二糖，而聚集蛋白聚糖的硫酸软骨素链含有25%的非硫酸化和70%的4-硫酸化。在软骨细胞N1511细胞分化的早期阶段，其中多功能蛋白聚糖的表达，连接蛋白过表达，增强多功能蛋白聚糖在基质中的沉积，并防止随后的聚集蛋白聚糖沉积。这些结果表明，多功能蛋白聚糖是目前作为一个不同于聚集蛋白聚糖聚集体的聚集体，并可能发挥特定的作用，在关节面。
Versican/PG-M is a large chondroitin sulfate proteoglycan of the extracellular matrix with a common domain structure to aggrecan and is present in cartilage at low levels. Here, we characterized cartilage versican during development and growth. Immunostaining showed that versican was mainly localized in the interterritorial zone of the articular surface at 2 weeks in mice, whereas aggrecan was in the pericellular zone of prehypertrophic and hypertrophic cells of the growth plate. Although its transcription level rapidly diminished during growth, versican remained in the articular cartilage. Biochemical analysis of normal articular cartilage and aggrecan-null cartilage from cmd (cartilage matrix deficiency)/cmd mice revealed that versican was present as a proteoglycan aggregate with both link protein and hyaluronan. Chondroitin sulfate chains of versican digested with chondroitinase ABC contained 71% nonsulfated and 28% 4-sulfated unsaturated disaccharides, whereas those of aggrecan contained 25% nonsulfated and 70% 4-sulfated. Link protein overexpression in chondrocytic N1511 cells at the early stage of differentiation, in which versican is expressed, enhanced versican deposition in the matrix and prevented subsequent aggrecan deposition. These results suggest that versican is present as an aggregate distinct from the aggrecan aggregate and may play specific roles in the articular surface.