Dynamics of Completely Unfolded and Native Proteins through Solid-State Nanopores as a Function of Electric Driving Force
Dynamics of Completely Unfolded and Native Proteins through Solid-State Nanopores as a Function of Electric Driving Force
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DOI：
10.1021/nn1034795
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发表时间：
2011-05-01
期刊：
影响因子：
17.1
通讯作者：
Pelta, Juan
中科院分区：
文献类型：
--
作者：
Oukhaled, Abdelghani;Cressiot, Benjamin;Pelta, Juan
我们实验报告的动态特性的入口和运输的未折叠和天然蛋白质通过固态纳米孔作为施加电压的函数，我们讨论的实验数据相比，理论。我们显示了一个指数增加的事件频率的电流封锁和运输时间的函数的电驱动力的指数下降。归一化的电流阻断比保持恒定或减少折叠或未折叠的蛋白质，分别作为跨膜电位的函数。未折叠的蛋白质在电驱动力下被拉伸。天然致密蛋白质在孔中的停留时间比未折叠蛋白质的停留时间长近1个数量级，并且两种蛋白质构象的事件频率都很低。我们讨论了阻碍蛋白质通过孔的运输的可能现象，这可以解释这些异常的动力学，特别是，电渗透逆流和蛋白质吸附在纳米孔壁上。
We report experimentally the dynamic properties of the entry and transport of unfolded and native proteins through a solid-state nanopore as a function of applied voltage, and we discuss the experimental data obtained as compared to theory. We show an exponential increase In the event frequency of current blockades and an exponential decrease in transport times as a function of the electric driving force. The normalized current blockage ratio remains constant or decreases for folded or unfolded proteins, respectively, as a function of the transmembrane potential. The unfolded protein is stretched under the electric driving force. The dwell time of native compact proteins in the pore is almost 1 order of magnitude longer than that of unfolded proteins, and the event frequency for both protein conformations is low. We discuss the possible phenomena hindering the transport of proteins through the pores, which could explain these anomalous dynamics, in particular, electro-osmotic counterflow and protein adsorption on the nanopore wall.