Regional assignment of the human uroporphyrinogen III synthase (UROS) gene to chromosome 10q25.2----q26.3.
Regional assignment of the human uroporphyrinogen III synthase (UROS) gene to chromosome 10q25.2----q26.3.
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人尿卟啉原 III 合酶 (UROS) 基因在染色体 10q25.2----q26.3 上的区域分配。
DOI：
10.1007/bf01213085
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发表时间：
1991
期刊：
影响因子：
5.3
通讯作者：
Desnick,RJ
中科院分区：
文献类型：
--
作者：
Astrin,KH;Warner,CA;Yoo,HW;Goodfellow,PJ;Tsai,SF;Desnick,RJ
尿卟啉原III合成酶[UROS；羟甲基双环丙烷水解酶，EC 4.2.1.75]是人类血红素生物合成途径中的第四种酶。最近分离到的编码人尿路蛋白的基因为其染色体定位提供了便利。通过聚合酶链式反应(PCR)从两组独立的人-啮齿类体细胞杂交的基因组DNA中特异性地扩增出人的Uros序列。人Uros扩增产物与人类10号染色体的符合率为100%，其余每条染色体与人Uros的不符合率均为19%~53%。对含有全长Uros基因的体细胞杂交种的DNA进行Southern杂交分析，证实了染色体定位。利用含有人类10号染色体不同部分的人-啮齿动物杂交种，我们将UROS基因分配到10q25.2→q26.3区域。
Uroporphyrinogen III synthase [UROS; hydroxymethylbilane hydro-lyase (cyclizing), EC 4.2.1.75] is the fourth enzyme in the human heme biosynthetic pathway. The recent isolation of the cDNA encoding human UROS facilitated its chromosomal localization. Human UROS sequences were specifically amplified by the polymerase chain reaction (PCR) from genomic DNA of two independent panels of human-rodent somatic cell hybrids. There was 100% concordance for the presence of the human UROS PCR product and human chromosome 10. For each of the other chromosomes, there was 19%–53% discordance with human UROS. The chromosomal assignment was confirmed by Southern hybridization analysis of DNA from somatic cell hybrids with the full-length UROS cDNA. Using human-rodent hybrids containing different portions of human chromosome 10, we assigned the UROS gene to the region 10q25.2→ q26.3.