Oral administration of Bifidobacterium longum prevents gut-derived Pseudomonas aeruginosa sepsis in mice
Oral administration of Bifidobacterium longum prevents gut-derived Pseudomonas aeruginosa sepsis in mice
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DOI：
10.1111/j.1365-2672.2007.03593.x
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发表时间：
2008-03-01
影响因子：
4
通讯作者：
Yamaguchi, K.
中科院分区：
文献类型：
--
作者：
Matsumoto, T.;Ishikawa, H.;Yamaguchi, K.
目的：本研究的目的是评估益生菌对免疫功能低下小鼠中铜绿假单胞菌引起的肠源性脓毒症的疗效。方法和结果：口服铜绿假单胞菌后，用环磷酰胺处理小鼠，诱导白细胞减少和肠道铜绿假单胞菌移位到血液中，从而产生肠源性脓毒症。在该模型中，与施用短双歧杆菌菌株ATCC 15700或益生菌粉末中所含赋形剂的小鼠组相比，施用1 × 10（9）CFU长双歧杆菌菌株BB 536 10天显著（P < 0.01）增加了存活率。施用B。与其他组相比，长双歧杆菌显著降低了肝脏和血液中铜绿假单胞菌的活菌计数。肠内容物培养显示B空肠中铜绿假单胞菌的活菌计数显著较低。longum处理的小鼠与其他小鼠组相比。此外，体外数据表明，B。结果表明，长双歧杆菌对Caco-2细胞的粘附活性明显高于其他各组，并能显著抑制铜绿假单胞菌对Caco-2细胞的粘附。结论：长双歧杆菌对小鼠肠源性脓毒症有保护作用，其作用机制可能与其干扰铜绿假单胞菌对肠上皮细胞的粘附有关。longum BB 536可有效防止耐药细菌如铜绿假单胞菌的机会性感染。结果表明，益生菌可能发挥重要作用，即使在免疫功能低下的患者。
Aims: The aim of the study was to evaluate the efficacy of probiotics on gut-derived sepsis caused by Pseudomonas aeruginosa in immunocompromised mice.Methods and Results: After oral inoculation of P. aeruginosa, mice were treated with cyclophosphamide to induce leucopenia and translocation of the intestinal P. aeruginosa into blood, thereby producing gut-derived sepsis. In this model, administration of 1 x 10(9) CFU of Bifidobacterium longum strain BB536 for 10 days significantly (P < 0.01) increased the survival rate compared with groups of mice administered either with Bifidobacterium breve strain ATCC 15700 or excipients contained in the probiotic bacterial powder. Administration of B. longum significantly decreased viable counts of P. aeruginosa in the liver and blood compared with other groups. Culture of intestinal contents revealed a significantly lower viable count of P. aeruginosa in the jejunum of B. longum-treated mice compared with other groups of mice. Furthermore, in vitro data demonstrated that B. longum possessed apparently higher adherent activity to Caco-2 cell monolayers and significantly suppressed the adherence of P. aeruginosa to the monolayers of cells compared with other groups.Conclusion: Oral administration of B. longum protects mice against gut-derived sepsis caused by P. aeruginosa, and the effect may be due to interference of P. aeruginosa adherence to intestinal epithelial cells.Significance and Impact of this Study: This study demonstrated that oral administration of B. longum BB536 is effective to protect against opportunistic infection with drug-resistant bacteria such as P. aeruginosa. The results suggest that probiotics may play an important role even in the immunocompromised patients.