Visualization of FtsZ Rings in Plastids of the Microspore in Lilium longiflorum
Visualization of FtsZ Rings in Plastids of the Microspore in Lilium longiflorum
复制标题
长花百合小孢子质体中 FtsZ 环的可视化
DOI：
10.1508/cytologia.66.113
复制
发表时间：
2001
期刊：
影响因子：
1
通讯作者：
T. Kuroiwa
中科院分区：
文献类型：
--
作者：
Toshiyuki Mori;M. Takahara;S. Miyagishima;H. Kuroiwa;T. Kuroiwa
先前的研究表明，在长花百合花粉生殖细胞中特异性表达的ftsZ基因在小孢子形成之前也在小孢子形成过程中表达。因此，我们用特异性抗体证明了L. longiflorum FtsZ （LlFtsZ）在无核小孢子中的表达。结果，在幼小孢子的细胞质中可见环。此外，通过4'，6-二氨基-2-苯基吲哚（DAPI）染色，发现几乎所有的环都重叠在每个质体上。每个球形和花生形状的质体在其赤道上有一个环。这些结果表明FtsZ环是质体分裂所必需的。
Previous study demonstrated that the ftsZ gene expressed specifically in pollen generative cells of Lilium longiflorum was also found to be expressed during microsporogenesis before formation of them. We, therefore, proved the expression of L. longiflorum FtsZ (LlFtsZ) in uninucleate microspores with specific antibodies. As a result, rings were visualized in the cytoplasm of young microspores. Moreover, it was revealed that almost all rings overlapped on each of plastids by 4', 6-diamidino-2-phenylindole (DAPI) staining. Each spherical and peanut-shaped plastid had one ring on its equator. These results suggest that FtsZ ring is required for plastid division.