Identification of protein-coding and intronic noncoding RNAs down-regulated in clear cell renal carcinoma
Identification of protein-coding and intronic noncoding RNAs down-regulated in clear cell renal carcinoma
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DOI：
10.1002/mc.20433
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发表时间：
2008-10-01
影响因子：
4.6
通讯作者：
Reis, Eduardo M.
中科院分区：
文献类型：
--
作者：
Brito, Glauber Costa;Fachel, Angela A.;Reis, Eduardo M.
肾细胞癌（RCC）的透明细胞亚型是泌尿系统最致命和最普遍的癌症。为了研究与透明细胞肾细胞癌恶性转化相关的分子变化，从6例患者中获得了肿瘤和邻近非肿瘤组织的匹配样本的基因表达谱。使用定制的cDNA微阵列平台，其包括2292个映射到基因外显子的探针和822个映射到内含子区域的非编码RNA的探针。在所有正常和肿瘤肾组织中均检测到内含子转录。通过两个统计检验和留一法患者交叉验证的组合确定，相对于匹配的非肿瘤组织，透明细胞RCC中55个转录物的子集显著下调。在下调的转录本中，49个定位于非翻译或编码外显子，6个相对于蛋白质编码基因的已知外显子为内含子。SIN 3B、TRIP 3、SYN J2 BP和NDE 1的表达水平较低（P
The clear cell subtype of renal cell carcinoma (RCC) is the most lethal and prevalent cancer of the urinary system. To investigate the molecular changes associated with malignant transformation in clear cell RCC, the gene expression profiles of matched samples of tumor and adjacent non-neoplastic tissue were obtained from six patients. A custom-built cDNA microarray platform was used, comprising 2292 probes that map to exons of genes and 822 probes for noncoding RNAs mapping to intronic regions. Intronic transcription was detected in all normal and neoplastic renal tissues. A subset of 55 transcripts was significantly down-regulated in clear cell RCC relative to the matched nontumor tissue as determined by a combination of two statistical tests and leave-one-out patient cross-validation. Among the down-regulated transcripts, 49 mapped to untranslated or coding exons and 6 were intronic relative to known exons of protein-coding genes. Lower levels of expression of SIN3B, TRIP3, SYNJ2BP and NDE1 (P