Evidence for two physiologically distinct gap junctions expressed by the chick lens epithelial cell.
Evidence for two physiologically distinct gap junctions expressed by the chick lens epithelial cell.
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DOI：
10.1083/jcb.102.1.194
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发表时间：
1986-01
期刊：
影响因子：
--
通讯作者：
Goodenough DA
中科院分区：
文献类型：
--
作者：
Miller TM;Goodenough DA
晶状体上皮细胞与两种不同的细胞类型交流。它们通过其外侧膜上的间隙连接与其他上皮细胞交流，并通过其顶端的连接与纤维细胞交流。我们独立测试了这两种细胞-细胞通信途径，以确定用90%二氧化碳平衡的介质处理是否会导致连接通透性降低；荧光染料的转移是测定方法。我们发现，高co2处理阻断了上皮内染料转移，但没有阻断纤维到上皮的染料转移。晶状体上皮细胞因此形成至少两类生理上不同的间隙连接。
Lens epithelial cells communicate with two different cell types. They communicate with other epithelial cells via gap junctions on their lateral membranes, and with fiber cells via junctions on their apices. We tested independently these two routes of cell-cell communication to determine if treatment with a 90% CO2-equilibrated medium caused a decrease in junctional permeability; the transfer of fluorescent dye was used as the assay. We found that the high-CO2 treatment blocked intraepithelial dye transfer but not fiber-to-epithelium dye transfer. The lens epithelial cell thus forms at least two physiologically distinct classes of gap junctions.