Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications
Assessment of Surface Glycan Diversity on Extracellular Vesicles by Lectin Microarray and Glycoengineering Strategies for Drug Delivery Applications
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DOI：
10.1002/smtd.202100785
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发表时间：
2021-12-01
期刊：
影响因子：
12.4
通讯作者：
Akiyoshi, Kazunari
中科院分区：
文献类型：
--
作者：
Shimoda, Asako;Miura, Risako;Akiyoshi, Kazunari
所有类型的哺乳动物细胞都释放细胞外囊泡 (EV)，用于细胞间通讯。在这项研究中，比较了 EV 上的表面聚糖的细胞类型、大小和分离方法，以检查凝集素微阵列的 EV 聚糖谱是否可用于定义 EV 亚群。此外，电动汽车采用四种独特的表面聚糖模式进行糖基工程，并评估其细胞摄取效率以用于潜在的药物输送应用。在每个实验条件下获得的 EV 上都鉴定了聚糖模式的相似性和差异。观察到 EV 大小和分离方法依赖性凝集素结合模式。此外，EV 的细胞摄取行为受到 EV 聚糖谱和受体细胞的影响。 EV 的体内生物分布也取决于其聚糖特征。这些结果表明，EV 表面聚糖是 EV 异质性的潜在新指标，而糖工程是调节生物医学应用中细胞-EV 相互作用的有用方法。
Extracellular vesicles (EVs) are released by all types of mammalian cells for cell-cell communication. In this study, surface glycans on EVs are compared in terms of their cell type, size, and isolation method to examine whether EV glycan profiles by lectin microarray can be used to define EV subpopulations. Moreover, EVs are glycoengineered with four distinctive surface glycan patterns and evaluated their cellular uptake efficiencies for potential drug delivery applications. Both similarities and differences in glycan patterns are identified on EVs obtained under each experimental condition. EV size- and isolation method-dependent lectin-binding patterns are observed. Moreover, cellular uptake behaviors of EVs are affected by EV glycan profiles and acceptor cells. The in vivo biodistribution of EVs is also dependent on their glycan profile. These results suggest that EV surface glycans are a potential novel indicator of EV heterogeneity, and glycoengineering is a useful approach to regulate cell-EV interactions for biomedical applications.