Development of an integrated pretreatment fractionation process for fermentable sugars and lignin: Application to almond (Prunus dulcis) shell
Development of an integrated pretreatment fractionation process for fermentable sugars and lignin: Application to almond (Prunus dulcis) shell
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开发可发酵糖和木质素的集成预处理分馏工艺：在杏仁 (Prunus dulcis) 壳中的应用
DOI：
10.1016/j.biombioe.2011.08.022
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发表时间：
2011-10-15
影响因子：
6
通讯作者：
Zhao, Ruming
中科院分区：
文献类型：
--
作者：
Gong, Dachun;Holtman, Kevin M.;Zhao, Ruming
采用热水预处理（HWP）和有机溶剂（organosolv）水/乙醇预处理（OWEP）相结合的方法，研究了从杏仁壳中分离纤维素、半纤维素和木质素的环保型预处理工艺。在可发酵糖和木质素分离产率方面，该综合预处理工艺比单独HWP、稀酸预处理（DAP）、氨预处理（AP）、石灰预处理LP、有机溶剂水/乙醇预处理（OWEP）和有机溶剂水/丙酮预处理（OWAP）更有效。在热水-有机溶剂耦合过程中，半纤维素糖在第一次残液中被回收，而在残液中保留了不同数量的纤维素。通过简单地调整第二种液体的pH值，得到木质素馏分。最佳的两段工艺为第一段HWP工艺，温度为195℃，时间为30 min，葡萄糖回收率为95.4%，木糖去除率为92.2%。第二个有机溶剂OWEP阶段在195℃下操作20分钟，在phi（乙醇）= 50%的乙醇和水的混合物中，获得了接近omega（葡萄糖）= 100%葡萄糖的回收率，omega（木糖）= 90%木糖和omega（木质素）= 61%木质素的去除率。经酶解后，葡萄糖的产率高达omega（葡萄糖）= 95%，而未经处理的杏仁产率为61%。通过扫描电镜（SEM）获得的图像突出了生物质分馏过程中不同预处理方法在杏仁结构上的差异。(C) 2011 Elsevier Ltd.版权所有。
An environmentally friendly pretreatment process was developed to fractionate cellulose, hemicellulose and lignin from almond (Prunus dulcis) shells, consisting of hot water pretreatment (HWP) coupled with organic solvent (organosolv) pretreatment of water/ethanol (OWEP). This integrated pretreatment process proved more effective on the basis of yield of fermentable sugar and lignin separation compared with HWP alone, dilute acid pretreatment (DAP), ammonia pretreatment (AP), lime pretreatment LP, organosolv water/ethanol pretreatment (OWEP), and organosolv water/acetone pretreatment (OWAP). In the coupled hot water-organosolv process, hemicellulose sugars were recovered in the first residual liquid while varying amounts of cellulose was retained in the residual solid. The lignin fraction was obtained by simply adjusting the pH from the second liquid. The optimal two-stage process consisted of first HWP stage at 195 degrees C for 30 min, resulting in omega(glucose) = 95.4% glucose recovery yield and omega(xylose) = 92.2% xylose removal. The second organosolv OWEP stage was operated at 195 degrees C for 20 min, in ethanol in water mixtures of phi(ethanol) = 50% and resulted in nearly omega(glucose) = 100% glucose recovery yield, omega(xylose) = 90% xylose and omega(lignin) = 61% lignin removal. After enzymatic hydrolysis, glucose yield was up to omega(glucose) = 95%, compared to 61% yield from untreated almond. Images obtained via scanning electron microscopy (SEM) highlighted the differences in almond structure from the varying pretreatment methods during biomass fractionation. (C) 2011 Elsevier Ltd. All rights reserved.