Molecular characterization of H9N2 influenza viruses: Were they the donors of the "internal" genes of H5N1 viruses in Hong Kong?
Molecular characterization of H9N2 influenza viruses: Were they the donors of the "internal" genes of H5N1 viruses in Hong Kong?
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DOI：
10.1073/pnas.96.16.9363
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发表时间：
1999-08-03
影响因子：
11.1
通讯作者：
Webster, RG
中科院分区：
文献类型：
--
作者：
Guan, Y;Shortridge, KF;Webster, RG
1997 年，H5N1 流感病毒导致 18 名感染者中的 6 人死亡，并且对鸡具有高致病性，其起源尚未解决。这些 H5N1 病毒从受感染的家禽直接传播给人类。在香港的家禽市场中，H5N1和H9N2流感病毒同时流行，增加了遗传重配的可能性，这里我们分析了不同流行病学背景的H9N2流感病毒的抗原和遗传特征，结果表明家鸭的H9N2流感病毒已经在亚洲的家禽中建立，从香港市场分离的H9N2病毒的系统发育和抗原分析表明存在三个不同的亚系，其中鸡H9N2病毒，其中6个基因片段显然源自中国分离的较早的鸡H9N2病毒，而PB1和PB2基因与H5N1病毒和鹌鹑H9N2病毒的基因密切相关——A/quail/香港/G1/97（Qa/HK/G1/97）——这表明市场上的许多1997年鸡H9分离株是重配体，内部的相似性Qa/HK/G1/97 病毒的基因与 H5N1 流感病毒的基因的比较表明，鹌鹑病毒可能是内部基因供体。我们的研究结果表明，1997年香港的人和家禽H5N1流感病毒是从Qa/HK/G1/97获得内部基因片段的重配体，但我们无法确定H5N1的复制复合体是否源自Qa/HEC/G1/97或是否发生了反向转移；现有证据支持前一项提议。
The origin of the H5N1 influenza viruses that killed six of eighteen infected humans in 1997 and were highly pathogenic in chickens has not been resolved. These H5N1 viruses transmitted directly to humans from infected poultry. In the poultry markets in Hong song, both H5N1 and H9N2 influenza viruses were cocirculating, raising the possibility of genetic reassortment, Here we analyze the antigenic and genetic features of H9N2 influenza viruses with different epidemiological backgrounds, The results suggest that the H9N2 influenza viruses of domestic ducks have become established in the domestic poultry of Asia, Phylogenetic and antigenic analyses of the H9N2 viruses isolated from Hong Kong markets suggest three distinct sublineages, Among the chicken H9N2 viruses, six of the gene segments were apparently derived from an earlier chicken H9N2 virus isolated in China, whereas the PB1 and PB2 genes are closely related to those of the H5N1 viruses and a quail H9N2 virus-A/quail/Hong Kong/G1/97 (Qa/HK/G1/97)-suggesting that many of the 1997 chicken H9 isolates in the markets were reassortants, The similarity of the internal genes of Qa/HK/G1/97 virus to those of the H5N1 influenza viruses suggests that the quail virus may have been the internal gene donor. Our findings indicate that the human and poultry H5N1 influenza viruses in Hong Kong in 1997 were reassortants that obtained internal gene segments from Qa/HK/G1/97, However, we cannot be certain whether the replicate complex of H5N1 originated from Qa/HEC/G1/97 or whether the reverse transfer occurred; the available evidence supports the former proposal.