TRANSMISSIBLE GASTROENTERITIS OF SWINE - VIRUS-INTESTINAL CELL INTERACTIONS .2. ELECTRON MICROSCOPY OF EPITHELIUM IN ISOLATED JEJUNAL LOOPS
TRANSMISSIBLE GASTROENTERITIS OF SWINE - VIRUS-INTESTINAL CELL INTERACTIONS .2. ELECTRON MICROSCOPY OF EPITHELIUM IN ISOLATED JEJUNAL LOOPS
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DOI：
10.1007/bf01253768
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发表时间：
1970-01-01
期刊：
影响因子：
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通讯作者：
PENSAERT, M
中科院分区：
文献类型：
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作者：
PENSAERT, M
对 7 日龄猪通过手术分离的小肠袢覆盖绒毛的柱状细胞形态进行电子显微镜研究表明，这些细胞的精细结构与其他哺乳动物物种的细胞非常相似。当用 TGE 病毒感染环时，在这些吸收细胞的细胞质中可以看到直径为 75 至 88 mμ 的病毒颗粒。病毒通过胞饮作用被吸收，新产生的颗粒通过在光滑的细胞膜（尤其是高尔基体）中出芽而成熟。细胞形态变化首先在微绒毛边界处检测到，并且似乎与病毒释放同时发生。随后通过脱皮和细胞破碎发生细胞损失。接种后18小时，萎缩的绒毛几乎完全被新产生的立方形细胞覆盖，其超微结构类似于未分化的隐窝细胞。这些细胞在接下来的几天和接种后 72 小时内逐渐分化，它们在形态上与正常吸收细胞相似。一些正在分化和分化的细胞被发现含有病毒颗粒。
Electron microscopy studies on the morphology of the columnar cells covering the villi in small intestinal loops, which were surgically isolated in 7 day old pigs, showed that the fine structure of those cells is very similar to that in other mammalian species. Upon infection of the loops with TGE virus, virus particles with a diameter of 75 to 88 mμ became visible in the cytoplasm of these absorptive cells. The virus was taken up by pinocytosis, and newly produced particles maturated by budding at smooth cellular membranes particularly in the Golgi apparatus. Cellular morphological changes were first detectable at the microvillar border and appeared to develop concomitantly with virus release. Loss of cells occurred later through desquamation and cell disruption. Atrophied villi were, at 18 hours postinoculation, almost completely covered with newly produced cuboidal cells which ultrastructurally resembled the undifferentiated crypt cells. Gradual differentiation of these cells occured during the following days and at 72 hours postinoculation, they were morphologically similar to normal absorptive cells. A few of the differentiating and differentiated cells were seen to contain virus particles.