Activation of leptin expression by an inhibitor of carnitine palmitoyltransferase-1.
Activation of leptin expression by an inhibitor of carnitine palmitoyltransferase-1.
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肉碱棕榈酰转移酶 1 抑制剂激活瘦素表达。
DOI：
10.1038/sj.ijo.0802585
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Urthaler,F
中科院分区：
文献类型：
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作者：
Wolkowicz,P;Grenett,H;Belousova,M;Urthaler,F
瘦素可能调节外周脂肪酸氧化，并激活影响脂肪细胞中瘦素表达的反馈机制。因此，本研究的目的是确定在肉碱棕榈酰基转移酶-1 （CPT1）水平上抑制全身脂肪酸氧化是否会影响瘦素的表达。为了实现这一目标，喂食或禁食的大鼠给予2-十四烷基甘糖酸（TDGA），一种特异性的、不可逆的CPT1抑制剂，并使用Northern、RT-PCR和放射免疫分析测量大鼠附睾瘦素表达和血清瘦素含量的急性变化。夜间禁食可降低附睾瘦素mRNA含量和血清瘦素水平。用TDGA处理过夜禁食大鼠，其附睾瘦素mRNA和血清瘦素均呈时间和浓度依赖性显著升高。TDGA既不影响全身脂肪酸氧化低的大鼠附睾瘦素mRNA，也不影响血清瘦素。这些结果支持了cpt1相关脂肪酸氧化是禁食大鼠瘦素表达的关键调节剂的结论。
Leptin may regulate peripheral fatty acid oxidation and invoke a feedback mechanism that affects leptin expression in adipocytes. The objective of this study, therefore, was to determine whether inhibiting systemic fatty acid oxidation at the level of carnitine palmitoyltransferase-1 (CPT1) affects leptin expression. To accomplish this objective, fed or overnight fasted rats were treated with 2-tetradecylglycidic acid (TDGA), a specific, irreversible CPT1 inhibitor, and acute changes in rat epididymal leptin expression and serum leptin content were measured using Northern, RT–PCR, and radioimmunoassay analyses. Overnight fasting decreased both epididymal leptin mRNA content and serum leptin. Treating overnight fasted rats with TDGA increased both their epididymal leptin mRNA and their serum leptin significantly in a time-and concentration-dependent manner. TDGA affected neither epididymal leptin mRNA nor serum leptin in fed rats where systemic fatty acid oxidation is low. These results support the conclusion that CPT1-linked fatty acid oxidation is a key modulator of leptin expression in fasting rats.