Effects of calf intestinal alkaline phosphatase, phosphatase inhibitors, and phosphorylated compounds on the rate of activation of glucocorticoid-receptor complexes.
Effects of calf intestinal alkaline phosphatase, phosphatase inhibitors, and phosphorylated compounds on the rate of activation of glucocorticoid-receptor complexes.
复制标题
小牛肠道碱性磷酸酶、磷酸酶抑制剂和磷酸化化合物对糖皮质激素受体复合物激活率的影响。
DOI：
10.1021/bi00564a046
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发表时间：
1980
期刊：
影响因子：
2.9
通讯作者：
Litwack,G
中科院分区：
文献类型：
--
作者：
Barnett,CA;Schmidt,TJ;Litwack,G
Carol A. Barnett,***§Thomas J. Schmidt， 5和Gerald Litwack*摘要：研究了(1)犊牛肠道碱性磷酸酶、(2)磷酸酶抑制剂和(3)磷酸化化合物对大鼠肝脏和肾脏糖皮质激素受体复合物激活率的影响。通过测量[3H]曲安奈德曲安奈德受体复合物与dna -纤维素的结合来定量活化程度，并通过DEAE-Sephadex A-50或deae -纤维素上未活化和活化的3H标记类固醇受体复合物的色谱分离来可视化。犊牛肠道碱性磷酸酶的添加可刺激大鼠肝细胞质中[3H]曲安奈德酮乙酰-奈德受体复合物的活化速率（0℃，2 h）。外源酶的热变性（90℃，15分钟）或竞争性抑制剂砷酸钠的加入可以阻止碱性磷酸酶介导的刺激。此外，一些磷酸酶抑制剂（钼酸盐和钨酸盐）可以阻断大鼠肝脏和肾脏细胞质中活化的[3H]曲安奈德受体复合物（25℃，30分钟）的形成，但不能
Carol A. Barnett,*** § Thomas J. Schmidt, 5 and Gerald Litwack* abstract: The effects of (1) calf intestinal alkaline phosphatase,(2) phosphatase inhibitors, and (3) phosphorylated compounds on the rate of activation of rat liver and kidney glucocorticoid-receptor complexes have been investigated. The extent of activation has been quantitated by measuring the binding of [3H] triamcinolone acetonide-receptor complexes to DNA-cellulose and visualized by chromatographic sepa-ration of unactivated and activated 3H-labeled steroid-receptor complexes on DEAE-Sephadex A-50 or DEAE-cellulose. The addition of calf intestinal alkaline phosphatase stimulates the rate of activation (0 C, 2 h) of [3H] triamcinolone aceto-nide-receptor complexes in rat liver cytosol. Heat denaturation (90 C, 15 min) of the exogenous enzyme or addition of the competitive inhibitor, sodium arsenate, prevents the alkaline phosphatase mediatedstimulation. In addition, formation of activated [3H] triamcinolone acetonide-receptor complexes (25 C, 30 min) in rat liver and kidney cytosols is blocked by some phosphatase inhibitors (molybdate and tungstate) but not by