Seeing double: a comparison of microstructure, biomechanical function, and adjacent disc health between double- and single-layer vertebral endplates.
Seeing double: a comparison of microstructure, biomechanical function, and adjacent disc health between double- and single-layer vertebral endplates.
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查看双重：比较双层和单层椎骨终结之间的微观结构，生物力学功能和相邻的椎间盘健康。
DOI：
10.1097/brs.0b013e318267bcfc
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发表时间：
2012-10-01
期刊：
影响因子：
3
通讯作者：
Lotz JC
中科院分区：
文献类型：
--
作者：
Fields AJ;Sahli F;Rodriguez AG;Lotz JC
从人尸体脊柱中采集的双层和单层骨终板样本的厚度、孔隙率、生物力学行为和相邻椎间盘糖胺聚糖（GAG）含量的实验和计算评估。确定双层椎体终板中的第二层骨是否允许浅层骨在其生物力学和营养功能之间实现更佳的平衡。适当的椎间盘健康需要终板平衡相反的生物力学和营养功能。以前的终板功能研究报道了双层终板的发生，但尚不清楚第二层骨是否具有任何功能优势。这些信息可以揭示防止椎间盘退变的因素。从人尸体（32-84岁）中获得六个腰椎，并使用磁共振成像进行扫描。从上级椎体终板（6个双层终板和12个单层终板）的中心采集包括终板和下层骨小梁的圆柱形核心，并使用micro-CT成像。测量每个芯骨的骨终板层的厚度和孔隙率。进行高分辨率有限元分析，以评估生物力学行为。使用二甲基亚甲蓝技术定量相邻核组织内的GAG含量。双层终板的表层比单层终板薄50%（p = 0.009），并且往往比单层终板更多孔。较薄终板的应变更高;然而，双层终板中的第二层骨具有硬化效应，因此尽管比单层终板薄，但双层终板的表层具有相似的损伤风险。调整年龄后，GAG含量在双层终板附近的核组织中显著较高（p = 0.01）。与单层终板相比，双层终板似乎允许终板生物力学和营养功能之间的更佳平衡，因此可能提供一个重要的保护因素，防止椎间盘退变。
Experimental and computational assessment of thickness, porosity, biomechanical behavior, and adjacent disc glycosaminoclycan (GAG) content in double-layer and single-layer bony endplate samples harvested from human cadaver spines. Determine if the second layer of bone in double-layer vertebral endplates allows the superficial layer to achieve a more optimal balance between its biomechanical and nutritional functions. Proper disc health requires the endplate to balance opposing biomechanical and nutritional functions. Previous studies investigating endplate function report the occurrence of double-layer endplates, but it remains unclear whether the second layer of bone has any functional advantage. Such information could shed light on the factors that protect against disc degeneration. Six lumbar spines were obtained from human cadavers (32–84 years) and scanned with magnetic resonance imaging. Cylindrical cores that included the endplate and underlying trabecular bone were harvested from the center of the superior vertebral endplates (six double-layer endplates and twelve single-layer endplates) and imaged using micro-CT. The thickness and porosity of the bony endplate layers was measured for each core. High-resolution finite element analysis was performed to assess biomechanical behavior. GAG content within the adjacent nucleus tissue was quantified using the dimethylmethylene blue technique. The superficial layer of the double-layer endplates was 50% thinner (p = 0.009) and tended also to be more porous than single-layer endplates. Strains were higher in thinner endplates; however, the second layer of bone in the double-layer endplates had a stiffening effect so that despite being thinner than single-layer endplates, the superficial layer of the double-layer endplates had a similar risk of damage. After adjusting for age, GAG content was significantly higher in the nucleus tissue adjacent to the double-layer endplates (p = 0.01). Compared to single-layer endplates, double-layer endplates appear to permit a more optimal balance between endplate biomechanical and nutritional function and may therefore offer a significant protective factor against disc degeneration.