High-performance liquid chromatographic method for determination of cefmetazole in human serum
High-performance liquid chromatographic method for determination of cefmetazole in human serum
复制标题
高效液相色谱法测定人血清中头孢美唑的含量
DOI：
10.1128/aac.21.5.740
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发表时间：
1982
影响因子：
4.9
通讯作者：
T. Morioka
中科院分区：
文献类型：
--
作者：
M. Sekine;K. Sasahara;T. Kojima;T. Morioka
建立了一种快速、特异、灵敏的高效液相色谱法测定人血清中头孢美唑浓度。通过加入含巴比妥作为内标物的5%三氯乙酸甲醇溶液对血清样品进行脱蛋白，并将其进样至反相色谱柱（mu-Bondapak C18）上，其中移动的流动相为10 - 15%乙腈的0.005 M柠檬酸盐缓冲液（pH 5.4）。通过254 nm处的UV吸收检测洗脱组分。用该法可将头孢美唑和内标物与干扰血清组分分离。头孢美唑与内标物的峰高比在0.4 - 100 μ g/ml范围内与头孢美唑浓度成正比。从三个病人单次静脉注射头孢美唑后获得的血清样品进行了测定，通过这种方法和微生物法。两种测定方法之间具有良好的相关性（相关系数，0.98）。头孢美唑在人血清中的稳定性为（r = 0.98）。用该方法测定了头孢美唑在人血清中的稳定性。头孢美唑在人血清中在4 ℃下稳定2周，如果冷冻保存，则至少稳定8周。由于高效液相色谱法具有简便、专属性强、准确、重现性好等优点，因此更适合于头孢菌素类抗生素的常规含量测定。
A fast, specific, sensitive high-performance liquid chromatographic method for the determination of cefmetazole in human serum was developed. The serum samples were deproteinized by adding 5% trichloroacetic acid in methanol containing barbital as an internal standard and were injected onto a reverse-phase column (mu-Bondapak C18) with a mobile phase of 10 to 15% acetonitrile in 0.005 M citrate buffer (pH 5.4). Eluted components were detected by UV absorption at 254 nm. Cefmetazole and the internal standard were separated from interfering serum components by this method. The peak height ratio of cefmetazole to the internal standard was proportional to the cefmetazole concentration in the range from 0.4 to 100 micrograms/ml. Serum samples obtained from three patients after a single intravenous injection of cefmetazole were assayed by this method and by a microbiological method. There was a good correlation between two assay methods (correlation, coefficient, 0.98). The stability of cefmetazole in human serum was (correlation coefficient, 0.98). The stability of cefmetazole in human serum was also determined by this method. Cefmetazole was stable in human serum for 2 weeks at 4 degrees C or for at least 8 weeks if it was kept frozen. As the high-performance liquid chromatography method is simple, specific, accurate, and reproducible, it appears to be more suitable for routine assay of cephalosporins than other assay methods.