Evaluation of fluorescence emitting characteristics of a microparticle by illumination angle scanning utilizing a resin-based monolithic TAS chip
Evaluation of fluorescence emitting characteristics of a microparticle by illumination angle scanning utilizing a resin-based monolithic TAS chip
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利用树脂基整体 TAS 芯片通过照明角度扫描评估微粒的荧光发射特性
DOI：
10.1007/s00542-016-3199-2
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Yoshikazu Yoshida
中科院分区：
文献类型：
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作者：
Toshifumi Ohkubo; Nobuyuki Terada; Yoshikazu Yoshida
光学传感器，例如光波导实现的全面分析系统（TAS），是在不久的将来实现“无处不在的人类医疗保健系统”所需的功能组件之一。我们已经提出了光波导的基本结构，该光波导能够照射沿微流体通道运行的活细胞或颗粒，并且甚至能够从微小颗粒体的极弱功率中检测荧光。为了培养方便地获取有关活细胞或颗粒的内部结构的信息的先进功能，顺序改变微小细胞或颗粒的照射方向的角度扫描方法可能是至关重要的。在本文中，我们通过使用新制造的具有独特交叉结构的 TAS 芯片切换激发激光源功率的照射方向，研究了附着在荧光物质上的树脂颗粒的荧光检测。为了经济有效地构建实验系统，我们采用了特殊的光波导结构，其中与流体通道的交叉点呈放射状布置，激光功率入口/出口部分平行布置。此外，我们在光纤顶端加入了强制振动机制，构建了一个简单的光开关系统。结合这种独特的TAS芯片和光学扫描机构的初步实验表明，径向排列的光波导切换功能充分发挥作用，并且准确评估了每个光波导的透射率变化。结果成功应用于检测到的角度扫描信号的补偿。最后，我们在数十毫米/秒（单次扫描）的较高交叉通过速度和数毫米/秒（多次扫描）的较低交叉通过速度的条件下，对附着在微粒上的荧光物质进行了角度扫描实验。基于获得的扫描数据，我们成功地构建了颗粒发射荧光的方向性，并阐明了荧光发射在入射光束的正向和正交方向上显示出其峰值。
An optical sensor, such as a light waveguide implemented total analysis system (TAS), is one of the functional components that will be needed to realize a “ubiquitous human healthcare system” in the near future. We have already proposed the fundamental structure for a light waveguide capable of irradiating a living cell or particle running along a microfluidic channel, and of detecting fluorescence even from the extremely weak power of a minute particulate body. In order to foster advanced functions for obtaining information concerning the internal structure of living cells or particles conveniently, an angular scanning method that sequentially changes the direction in which the minute cell or particle is irradiated may be crucial. In this paper, we investigate fluorescence detection from resin particles attached to a fluorescent substance by switching irradiation direction of the exciting laser source power using a newly fabricated TAS chip with a unique intersection structure. To construct the experimental system cost effectively, we adopted a special light waveguide structure in which the intersection to a fluidic channel was arranged radially, with laser power inlet/outlet portions arranged in parallel. In addition, we incorporated a forced vibration mechanism on an optical fiber apex to construct a simple light-switching system. Preliminary experiments combining this unique TAS chip and the optical scanning mechanism revealed that the function of the radially arranged light waveguide switching worked adequately, and variation of transmittance of each light waveguide was accurately evaluated. Results were successfully applied to compensation of detected angular scanning signals. Finally, we performed an angular scanning experiment for a fluorescent substance attached to a microparticle under conditions of higher intersection-passing velocity of several 10s mm/s (single-shot scanning) and lower intersection-passing velocity of several mm/s (multiple-shot scanning). Based on the obtained scanning data, we successfully constructed directivities of emitting fluorescence from particles, and clarified that fluorescence emission indicated its peak at the forward and orthogonal directions of the incident light beam.