ISOLATION AND CHARACTERIZATION OF 52 POLYMORPHIC EST-SSR MARKERS FOR CALLITRIS COLUMELLARIS (CUPRESSACEAE)
ISOLATION AND CHARACTERIZATION OF 52 POLYMORPHIC EST-SSR MARKERS FOR CALLITRIS COLUMELLARIS (CUPRESSACEAE)
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DOI：
10.3732/ajb.1100276
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发表时间：
2011-12-01
影响因子：
3
通讯作者：
Isagi, Yuji
中科院分区：
文献类型：
--
作者：
Sakaguchi, Shota;Uchiyama, Kentaro;Isagi, Yuji
本研究的目的：利用表达序列标签（ESTs）构建了柱萼马蹄莲（Callitris columellaris s.l.）方法和结果：对来自单个幼苗的mRNA进行cDNA合成，然后进行从头焦磷酸测序。从获得的序列数据设计了219个与序列区域接壤的引物对。结果表明，在16个个体中有52个位点表现出多态性，每个位点的等位基因数和期望杂合度分别为2 ~ 10和0.06 ~ 0.84。科列利亚里斯公司检测选择过程中的异常位点，为物种的保护遗传学和生殖生态学研究提供有用的标记。
Premise of the study: We developed simple sequence repeat (SSR) markers from expressed sequence tags (ESTs) for Callitris columellaris sensu lato (s.l.) to elucidate population genetic structure and detect outlier loci by genome scan.Methods and Results: mRNA from an individual seedling was subjected to cDNA synthesis and then de novo pyrosequencing. Two hundred and nineteen primer pairs bordering sequence regions were designed from the obtained sequence data. In total, 52 showed polymorphism within 16 individuals representative of the species' entire range, with the number of alleles per locus and expected heterozygosity ranging from two to 10 and 0.06 to 0.84, respectively.Conclusions: The EST-SSR markers developed in this study will be useful for evaluating the range-wide genetic structure of C. columellaris s.l. and detecting outlier loci under selection, as well as providing useful markers to investigate the conservation genetics and reproductive ecology of the species.