Inducing perylenequinone production from a bambusicolous fungus Shiraia sp. S9 through co-culture with a fruiting body-associated bacterium Pseudomonas fulva SB1
Inducing perylenequinone production from a bambusicolous fungus Shiraia sp. S9 through co-culture with a fruiting body-associated bacterium Pseudomonas fulva SB1
复制标题
诱导竹子真菌 Shiraia sp 产生苝醌。
DOI：
10.1186/s12934-019-1170-5
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发表时间：
2019-07-05
影响因子：
6.4
通讯作者：
Wang, Jian Wen
中科院分区：
文献类型：
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作者：
Ma, Yan Jun;Zheng, Li Ping;Wang, Jian Wen
背景：白念珠菌子实体中的苝醌色素是一种优良的光敏剂，在医学和农业上有广泛的应用。子实体由功能未知的多种细菌群落定殖。结果：从Shiraia sp. S9子实体中分离到一株细菌Pseudomonasfulva SB 1，该细菌能刺激真菌产生竹红菌素A、C（HA和HC）和痂囊腔菌素A-C（EA、EB和EC）。共培养2d后，白念珠菌菌丝体的HA产量最高（325.87mg/L），是纯培养的3.20倍。共培养导致真菌分生孢子的诱导和更紧凑的真菌球的形成。此外，细菌处理上调聚酮合成酶基因（PKS）的表达，并激活转运蛋白基因的ATP结合盒（ABC）和主要易化超家族转运蛋白（MFS）的PQ exudation.Conclusions：我们已经建立了细菌共培养与宿主Shiraia真菌诱导PQ的生物合成。我们的研究结果为了解子实体中细菌-真菌的相互作用和实际的共培养过程提供了基础，以提高光动力治疗药物的PQ生产。
Background:Fungal perylenequinonoid (PQ) pigments from Shiraia fruiting body have been well known as excellent photosensitizers for medical and agricultural uses. The fruiting bodies are colonized by a diverse bacterial community of unknown function. We screened the companion bacteria from the fruiting body of Shiraia sp. S9 and explored the bacterial elicitation on fungal PQ production.Results:A bacterium Pseudomonas fulva SB1 isolated from the fruiting body was found to stimulate the production of fungal PQs including hypocrellins A, C (HA and HC), and elsinochromes A-C (EA, EB and EC). After 2 days of co-cultures, Shiraia mycelium cultures presented the highest production of HA (325.87 mg/L), about 3.20-fold of that in axenic culture. The co-culture resulted in the induction of fungal conidiation and the formation of more compact fungal pellets. Furthermore, the bacterial treatment up-regulated the expression of polyketide synthase gene (PKS), and activated transporter genes of ATP-binding cassette (ABC) and major facilitator superfamily transporter (MFS) for PQ exudation.Conclusions:We have established a bacterial co-culture with a host Shiraia fungus to induce PQ biosynthesis. Our results provide a basis for understanding bacterial-fungal interaction in fruiting bodies and a practical co-culture process to enhance PQ production for photodynamic therapy medicine.