High presence of extended-spectrum beta-lactamases and resistance to quinolones in clinical isolates of Escherichia coli.
High presence of extended-spectrum beta-lactamases and resistance to quinolones in clinical isolates of Escherichia coli.
复制标题
大肠杆菌临床分离株中存在大量广谱 β-内酰胺酶和对喹诺酮类药物的耐药性。
DOI：
10.1016/j.micres.2006.02.003
复制
发表时间：
2007
影响因子：
1.5
通讯作者：
G. Piédrola
中科院分区：
文献类型：
--
作者：
A. Sorlózano;J. Gutiérrez;J. de Dios Luna;J. Oteo;J. Liébana;M. Soto;G. Piédrola
对706株大肠埃希菌进行了超广谱β-内酰胺酶（ESBLs）检测，其中75.2%来自门诊患者。使用临床和实验室标准化研究所（原NCCLS）推荐的纸片扩散法检测ESBL的存在;使用VITEK 2系统（bioMérieux，Marcy L 'Etoile，France）确定对临床感兴趣抗生素的敏感性，并通过生化研究、测定等电点和PCR分子研究表征ESBL。在8个医院分离株的克隆分布进行了研究。产ESBL菌株115株（16.3%），以CTX-M9型为主（58.3%）。我们注意到CTX-M9和SHV产酶菌株对喹诺酮类药物的高耐药性（>70%），后者的氨基糖苷类活性较低。
A study was conducted to detect the presence of extended-spectrum β-lactamases (ESBLs) in 706 isolates of Escherichia coli, largely from outpatients (75.2%). The Clinical and Laboratory Standards Institute (formerly NCCLS)-recommended disk diffusion procedure was used to detect ESBL presence; the VITEK 2 system (bioMérieux, Marcy L’Etoile, France) was used to determine the susceptibility to antibiotics of clinical interest, and the ESBLs were characterized by biochemical study, determining the isoelectric point, and by molecular study with PCR. Clonal distribution was studied in eight hospital isolates. There were 115 ESBL-producing isolates (16.3%), with a predominance of CTX-M9 type (58.3%). We draw attention to the high resistance to quinolones (>70%) in CTX-M9 and SHV enzyme producing isolates and the lower aminoglycoside activity in the latter.