Stability of interleukin 6, soluble interleukin 6 receptor, interleukin 10 and CC16 in human serum
Stability of interleukin 6, soluble interleukin 6 receptor, interleukin 10 and CC16 in human serum
复制标题
DOI：
10.1016/s1043-4666(02)91961-7
复制
发表时间：
2002-09-07
期刊：
影响因子：
3.8
通讯作者：
Maes, M
中科院分区：
文献类型：
--
作者：
Kenis, G;Teunissen, C;Maes, M
根据Arrhenius方程，采用加速稳定性试验方法检测了人血清中白细胞介素6(IL-6)、其可溶性受体(sIL-6R)、IL-10和CC16或子宫红蛋白(一种内源性细胞因子抑制物)的稳定性。此外，还测定了采血和样品处理之间的时间延迟、凝血温度和反复冻融循环对血清中这些蛋白质水平的影响。配对血清样品在4℃、20℃、30℃和40℃下保存1-21天。我们发现，IL-6和CC16浓度在4℃、20℃和30℃时没有变化。白介素6浓度在40摄氏度下11天后显著下降。SIL-6R和IL-10浓度在4℃时无明显变化，但在20℃(分别在21天和14天后)、30℃和40℃(两种温度下均为I天后)均显著下降。Arrhenius图表明，sIL-6R和IL-10分别在-20℃和-70℃下至少稳定数年。由于它们的相对稳定性，IL-6和CC16不能计算阿累尼乌斯曲线图。在4℃和20℃下，反复冻融循环和延长凝血时间对所检测蛋白质的浓度没有显著影响。我们认为，用于测定IL-6、sIL-6R和CC16的血清样品可以在-20℃保存数年，但对于IL-10测定，建议在-70℃保存。(C)2002爱思唯尔科学有限公司。保留所有权利。
The stability of interleukin 6 (IL-6), its soluble receptor (sIL-6R), IL-10 and CC16 or uteroglobin (an endogenous cytokine inhibitor) in human serum was examined using an accelerated stability testing protocol according to the Arrhenius equation. Further, the effect of time delay between blood sampling and sample processing, clotting temperature and repeated freeze-thaw cycles on serum levels of these proteins were determined. Paired serum samples were stored at 4degreesC, 20degreesC, 30degreesC and 40degreesC for 1 to 21 days. We found that IL-6 and CC16 concentrations did not change at 4degreesC, 20degreesC and 30degreesC. Interleukin-6 concentrations significantly declined after 11 days at 40degreesC. The concentrations of sIL-6R and IL-10 did not change at 4degreesC but significantly decreased at 20degreesC (after 21 and 14 days respectively), 30degreesC and 40degreesC (after I day at both temperatures for both cytokines). Arrhenius-plots indicated that sIL-6R and IL-10 are stable for at least several years at - 20degreesC and - 70degreesC, respectively. Since their relative stability, no Arrhenius-plot could be calculated for IL-6 and CC16. The concentrations of the proteins examined were not significantly altered by repeated freeze-thaw cycles, nor by extended clotting times at 4degreesC or 20'C. We conclude that serum samples for the determination of IL-6, sIL-6R and CC16 can be stored at - 20degreesC for several years, but for IL-10 determinations, storage at - 70degreesC is recommended. (C) 2002 Elsevier Science Ltd. All rights reserved.