mRNA expression of ligands of the epidermal‐growth‐factor‐receptor in the uterus
mRNA expression of ligands of the epidermal‐growth‐factor‐receptor in the uterus
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DOI：
10.1002/(sici)1097-0215(19970807)72:4
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发表时间：
1997-08
影响因子：
6.4
通讯作者：
D. Pfeiffer;J. Spranger;Michael Al‐Deiri;R. Kimmig;A. Fisseler‐Eckhoff;P. Scheidel;H. Schatz;Arne Jensen;A. Pfeiffer
中科院分区：
文献类型：
--
作者：
D. Pfeiffer;J. Spranger;Michael Al‐Deiri;R. Kimmig;A. Fisseler‐Eckhoff;P. Scheidel;H. Schatz;Arne Jensen;A. Pfeiffer
过去已经鉴定了表皮生长因子受体（EGFR）的六种不同配体。在一些宫颈鳞状细胞癌中，已报告与EGFR结合的蛋白质量增加。为了鉴定可能在宫颈癌和子宫内膜癌中过度表达的EGFR配体（EGFRL）的mRNA，我们对人子宫正常和恶性组织样本的RNA提取物中的所有6种EGFRL进行了半定量逆转录/聚合酶链反应（RT-PCR）。通过分析产物合成的PCR动力学，定量相对于管家基因和内标丙酮酸脱氢酶的83例患者的RNA提取物的PCR产物。在正常子宫颈组织中，EGFRL mRNA的表达水平明显高于子宫内膜组织。正常宫颈与宫颈癌之间无显著性差异。然而，在宫颈癌和子宫内膜癌中，mRNA表达呈非参数分布，在一些宫颈癌中观察到转化生长因子α（TGF-α）、双调蛋白或EGF过表达。在子宫内膜癌中，所有EGFRL的mRNA水平均高于正常子宫内膜。TGF-α和双调蛋白的这种增加是显著的（p < 0.005）。因此，TGF-α mRNA在大约10%的宫颈癌和大多数子宫内膜癌中过度表达。由于TGF-α反义治疗可能代表了此类癌症的未来策略，因此我们还使用克隆标准品通过定量PCR测定了TGF-α mRNA表达的绝对水平。Int. J. Cancer 72：581-586，1997.© 1997 Wiley利斯公司。
Six different ligands of the epidermal‐growth‐factor receptor (EGFR) have been identified in the past. In some cervical squamous‐cell carcinomas, an increased amount of proteins binding to the EGFR has been reported. In order to identify the mRNA of EGFR ligands (EGFRL), which might be over‐expressed in cervical and endometrial cancers, we performed semi‐quantitative reverse‐transcription/polymerase chain reactions (RT‐PCR) for all 6 EGFRL in RNA extracts of normal and malignant tissue samples of the human uterus. PCR products from RNA extracts of 83 patients were quantitated relative to the housekeeping gene and internal standard pyruvate dehydrogenase by analyzing the PCR kinetics of product synthesis. In extracts of normal cervix, the level of mRNA expression of the EGFRL was significantly higher than in endometrium. No significant difference was detected between normal cervix and cervical carcinomas. However, both in cervical and in endometrial cancers, mRNA expression was non‐parametrically distributed and in some cervical cancers overexpression of transforming growth factor alpha (TGF‐α), amphiregulin or EGF was observed. In endometrial cancers, mRNA levels of all EGFRL were higher than in normal endometrium. This increase was significant (p < 0.005) for TGF‐α and amphiregulin. Thus, TGF‐α mRNA is over‐expressed in approximately 10% of cervical cancers and in the majority of endometrial cancers. Since TGF‐α anti‐sense therapy might represent a future strategy in such cancers, we also determined the absolute level of TGF‐α mRNA expression by quantitative PCR using a cloned standard. Int. J. Cancer 72:581–586, 1997.© 1997 Wiley‐Liss, Inc.