Applications of 1.06-micron IR laser desorption on a Fourier transform mass spectrometer.
Applications of 1.06-micron IR laser desorption on a Fourier transform mass spectrometer.
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1.06 微米红外激光解吸在傅里叶变换质谱仪上的应用。
DOI：
10.1021/ac980914s
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发表时间：
1998
影响因子：
7.4
通讯作者：
C. Fenselau
中科院分区：
文献类型：
--
作者：
Y. Ho;C. Fenselau
在傅立叶变换质谱仪上使用激光解吸和电离在有和没有基质的帮助下分析各种糖、肽和脂质。采用了一种紧凑的Nd：YAG激光器，其输出为1.06微米，对应于基频。革兰氏阴性和革兰氏阳性细菌也进行了激光解吸质谱。观察到细胞内源性碱金属阳离子结合形成的共轭脂质的特征离子。颗粒/液体基质（例如，甘油中的钴）被证明可用于1.06微米激光。该颗粒有效地吸收宽波长范围内的激光辐射。该液体提供了与快速原子轰击相同的优势：提高信噪比和延长样品寿命。激光功率对总离子电流的影响被证明是不同的样品与无颗粒/液体基质。傅里叶变换分析仪提供MS/MS功能，可检测复杂混合物中的正离子和负离子。外部解吸的离子通过具有较低质量截止的四极离子导向器引入电池中。这样的设置允许基质离子被排除，并且因此为在单元内形成的较低质量范围碎片离子提供优异的信噪比。
Various sugars, peptides, and lipids were analyzed on a Fourier transform mass spectrometer using laser desorption and ionization with and without the assistance of matrixes. A compact Nd:YAG laser with an output at 1.06 microns corresponding to fundamental frequency was employed. Gram-negative and Gram-positive bacteria were also subjected to laser desorption mass spectrometry. Characteristics ions of conjugated lipid, formed by attachment of alkali metal cations, endogenous to the cells, were observed. Particle/liquid matrixes (e.g., cobalt in glycerol) proved to be useful with the 1.06-micron laser. The particles absorb efficiently laser radiation in a broad wavelength range. The liquid provides the same advantages as in fast atom bombardment: increased signal-to-noise ratios and enhanced sample lifetimes. The effect of laser power on total ion current was shown to differ for samples with and without the particle/liquid matrix. The Fourier transform analyzer provides MS/MS capability for both positive and negative ions from complex mixtures. Ions desorbed externally are introduced into the cell via a quadrupole ion guide with a lower mass cutoff. Such a setup allows matrix ions to be excluded and thus provides excellent signal-to-noise ratios for lower mass range fragment ions formed inside the cell.